Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2021 Mar 15;22(6):2972. doi: 10.3390/ijms22062972.
Seed coat color is an important agronomic trait of edible seed pumpkin in . In this study, the development pattern of seed coat was detected in yellow and white seed coat accessions Wuminglv and Agol. Genetic analysis suggested that a single recessive gene () is involved in seed coat color regulation in . An F segregating population including 2798 plants was used for fine mapping and a candidate region containing nine genes was identified. Analysis of 54 inbred accessions revealed four main Insertion/Deletion sites in the promoter of encoding an MYB transcription factor were co-segregated with the phenotype of seed coat color. RNA-seq analysis and qRT-PCR revealed that some genes involved in phenylpropanoid/flavonoid metabolism pathway displayed remarkable distinction in Wuminglv and Agol during the seed coat development. The flanking InDel marker S1548 was developed to predict the seed coat color in the MAS breeding with an accuracy of 100%. The results may provide valuable information for further studies in seed coat color formation and structure development in Cucurbitaceae crops and help the molecular breeding of .
种皮颜色是食用籽用南瓜的一个重要农艺性状。本研究在黄色和白色种皮材料‘无名绿’和‘Agol’中检测到种皮的发育模式。遗传分析表明，一个单隐性基因 () 参与调控南瓜的种皮颜色。利用包含 2798 株的 F2 分离群体进行精细定位，鉴定到一个包含 9 个基因的候选区域。对 54 份自交系的分析揭示，在编码 MYB 转录因子的基因启动子中存在四个主要的插入/缺失位点，与种皮颜色表型共分离。RNA-seq 分析和 qRT-PCR 显示，在种皮发育过程中，‘无名绿’和‘Agol’中一些参与苯丙烷类/类黄酮代谢途径的基因表现出明显的差异。开发了侧翼 InDel 标记 S1548 用于 MAS 育种中的种皮颜色预测，准确率为 100%。该结果可为进一步研究葫芦科作物种皮颜色形成和结构发育提供有价值的信息，并有助于 的分子育种。
