围产期大鼠肾上腺的儿茶酚胺含量。

The catecholamine content of the perinatal rat adrenal gland.

Coulter C L, McMillen I C, Browne C A

Department of Physiology, Monash University, Clayton, Victoria, Australia.

Gen Pharmacol. 1988;19(6):825-8.

Abstract

High performance liquid chromatography combined with electro-chemical detection (HPLC/ECD) has been used to measure the noradrenaline, adrenaline and dopamine content of individual adrenal glands from adult rats and from rats aged between day 16 of gestation and day 12 after birth. 2. At all ages studied, the dopamine content of the rat adrenal was significantly lower than the noradrenaline or adrenaline content. 3. Noradrenaline was detected at day 16 of gestation (7.58 ng/gland) and there was a 26 fold increase in the noradrenaline content of the rat adrenal by day 10 after birth (201.67 +/- 51.01 ng/gland). 4. Adrenaline was also detected at day 16 of gestation (2.19 ng/gland) and there was a 700 fold increase in adrenaline content of the rat adrenal by day 10 after birth (1408.62 +/- 473.00 ng/gland). 5. The adrenaline: noradrenaline ratio exceeded one by day 18 of gestation (1.60:1) and had increased to (3.05:1) at birth. 6. This ratio at day 10 after birth (7.43:1) was significantly greater than the ratio at or before birth, and also in the adult (4.20:1).

摘要

高效液相色谱结合电化学检测法（HPLC/ECD）已被用于测定成年大鼠以及妊娠第16天至出生后第12天大鼠单个肾上腺中去甲肾上腺素、肾上腺素和多巴胺的含量。2. 在所有研究的年龄段中，大鼠肾上腺中的多巴胺含量均显著低于去甲肾上腺素或肾上腺素含量。3. 在妊娠第16天检测到去甲肾上腺素（7.58纳克/腺体），到出生后第10天，大鼠肾上腺中的去甲肾上腺素含量增加了26倍（201.67±51.01纳克/腺体）。4. 在妊娠第16天也检测到了肾上腺素（2.19纳克/腺体），到出生后第10天，大鼠肾上腺中的肾上腺素含量增加了700倍（1408.62±473.00纳克/腺体）。5. 肾上腺素与去甲肾上腺素的比值在妊娠第18天超过1（1.60:1），出生时增加到（3.05:1）。6. 出生后第10天的该比值（7.43:1）显著高于出生时或出生前以及成年大鼠的比值（4.20:1）。