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PIC1 和 NiCo 的发育和铁营养模式不支持它们在油菜叶绿体铁转运中相互依存和排他的合作。

The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus.

机构信息

Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary.

RT-Europe Nonprofit Research Ltd., Mosonmagyaróvár, Hungary.

出版信息

Planta. 2020 Apr 15;251(5):96. doi: 10.1007/s00425-020-03388-0.

DOI:10.1007/s00425-020-03388-0
PMID:32297017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7214486/
Abstract

The accumulation of NiCo following the termination of the accumulation of iron in chloroplast suggests that NiCo is not solely involved in iron uptake processes of chloroplasts. Chloroplast iron (Fe) uptake is thought to be operated by a complex containing permease in chloroplast 1 (PIC1) and nickel-cobalt transporter (NiCo) proteins, whereas the role of other Fe homeostasis-related transporters such as multiple antibiotic resistance protein 1 (MAR1) is less characterized. Although pieces of information exist on the regulation of chloroplast Fe uptake, including the effect of plant Fe homeostasis, the whole system has not been revealed in detail yet. Thus, we aimed to follow leaf development-scale changes in the chloroplast Fe uptake components PIC1, NiCo and MAR1 under deficient, optimal and supraoptimal Fe nutrition using Brassica napus as model. Fe deficiency decreased both the photosynthetic activity and the Fe content of plastids. Supraoptimal Fe nutrition caused neither Fe accumulation in chloroplasts nor any toxic effects, thus only fully saturated the need for Fe in the leaves. In parallel with the increasing Fe supply of plants and ageing of the leaves, the expression of BnPIC1 was tendentiously repressed. Though transcript and protein amount of BnNiCo tendentiously increased during leaf development, it was even markedly upregulated in ageing leaves. The relative transcript amount of BnMAR1 increased mainly in ageing leaves facing Fe deficiency. Taken together chloroplast physiology, Fe content and transcript amount data, the exclusive participation of NiCo in the chloroplast Fe uptake is not supported. Saturation of the Fe requirement of chloroplasts seems to be linked to the delay of decomposing the photosynthetic apparatus and keeping chloroplast Fe homeostasis in a rather constant status together with a supressed Fe uptake machinery.

摘要

叶绿体中铁积累停止后镍钴的积累表明，镍钴不仅参与叶绿体的铁吸收过程。叶绿体铁（Fe）的摄取被认为是由叶绿体 1 中的渗透酶（PIC1）和镍钴转运蛋白（NiCo）蛋白组成的复合物来操作的，而其他铁稳态相关转运蛋白（如多抗生素耐药蛋白 1（MAR1））的作用则不太明确。尽管有一些关于叶绿体铁摄取的调节信息，包括植物铁稳态的影响，但整个系统尚未详细揭示。因此，我们旨在使用油菜作为模型，在缺铁、最佳和超最佳铁营养条件下，跟踪叶片发育过程中叶绿体铁摄取成分 PIC1、NiCo 和 MAR1 的变化。缺铁降低了光合作用活性和质体中的铁含量。超最佳铁营养既不会导致叶绿体中铁的积累，也不会产生任何毒性作用，因此只会完全满足叶片中铁的需求。随着植物铁供应的增加和叶片的衰老，BnPIC1 的表达被倾向地抑制。尽管 BnNiCo 的转录本和蛋白量在叶片发育过程中倾向于增加，但在衰老叶片中甚至明显上调。在缺铁的衰老叶片中，BnMAR1 的相对转录本量主要增加。总的来说，叶绿体生理学、铁含量和转录本数量的数据不支持 NiCo 排他性地参与叶绿体铁的摄取。叶绿体对铁需求的饱和似乎与分解光合作用装置的延迟以及保持叶绿体铁稳态的相对恒定状态有关，同时还抑制了铁摄取机制。

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