Voivodeship Specialist Hospital in Wloclawek, Wloclawek, Poland.
Faculty of Health Science, Public Higher Medical Professional School in Opole, Opole, Poland.
Curr Pharm Biotechnol. 2020;21(11):1119-1128. doi: 10.2174/1389201021666200416102540.
Semaphorin 3F (SEMA3F) plays a substantial role in carcinogenesis, because of its role in inducing angiogenesis, and creating a microenvironment for the developing tumor.
The purpose of this work was to assess the impact of cisplatin, depending on the concentration and exposure time on the expression pattern of SEMA3F in an endometrial cancer cell line.
Cultures of the Ishikawa endometrial cancer cells were incubated with cisplatin with the following concentrations: 2.5μM; 5μM; and 10μM and for the following periods of time: 12; 24; and 48 hours. Cells not incubated with the drug constituted the control in the experiment. To determine the effect of cisplatin on the expression of SEMA3F, the real-time quantitative reverse transcription reaction (RtqPCR; mRNA) was used, as well as the ELISA assay (protein). The statistical analysis was done with the admission of p<0.05.
The silencing of SEMA3F expression on the transcriptome and proteome levels in a culture unexposed to the effects of cisplatin in comparison to endometrial cancer cells under the influence of cisplatin (p<0.05) were noted. Along with an increase in the concentration of the drug used, the number of copies of the gene transcript, during the shortest incubation period had a gradual increase. Only for the highest concentration of the drug, substantial statistical differences in the expression of the SEMA3F protein between 24 and 48 hour incubation periods (p<0.05) were determined.
Using cisplatin in an endometrial cancer cell culture results in an increased expression of SEMA3F, which advantageously affects the normalization of the neoplastic angiogenic process and lowers the proliferation of the cells making up the mass of the tumor.
信号素 3F（SEMA3F）在致癌作用中起着重要作用，因为它在诱导血管生成和为肿瘤的发展创造微环境方面发挥作用。
本工作旨在评估顺铂浓度和暴露时间对子宫内膜癌细胞系中 SEMA3F 表达模式的影响。
用以下浓度的顺铂孵育 Ishikawa 子宫内膜癌细胞：2.5μM；5μM；和 10μM，并孵育以下时间：12；24；和 48 小时。未用药物孵育的细胞构成实验中的对照。为了确定顺铂对 SEMA3F 表达的影响，使用实时定量逆转录反应（RtqPCR；mRNA）和 ELISA 测定（蛋白质）。通过承认 p<0.05 进行统计分析。
在未暴露于顺铂作用的培养物中，与在顺铂作用下的子宫内膜癌细胞相比，在转录组和蛋白质组水平上观察到 SEMA3F 表达的沉默（p<0.05）。随着所用药物浓度的增加，在最短孵育时间内基因转录本的拷贝数逐渐增加。仅对于最高药物浓度，在 24 和 48 小时孵育期间，SEMA3F 蛋白表达之间存在显著的统计学差异（p<0.05）。
在子宫内膜癌细胞培养中使用顺铂会导致 SEMA3F 的表达增加，这有利于正常化肿瘤血管生成过程并降低构成肿瘤质量的细胞的增殖。
