Post-Graduation Program in Biomedical Engineering, Universidade Brasil, São Paulo, Brazil.
Laboratory of Physiology and Human Performance, São Paulo State University - UNESP, Bauru, São Paulo, Brazil.
J Photochem Photobiol B. 2020 Jun;207:111877. doi: 10.1016/j.jphotobiol.2020.111877. Epub 2020 Apr 7.
Photobiomodulation therapy (PBMT) has many effects on the energy metabolism of musculoskeletal tissue, such as increased glycogen and adenosine triphosphate synthesis. In addition, these effects may be due to a systemic blood glucose control. Twenty-four Wistar rats were randomly and equally allocated into four groups: sham, PBMT 10 J/cm, PBMT 30 J/cm and PBMT 60 J/cm. The animals were fasting for 6 h for blood glucose evaluations during pre-irradiation period, 1 h, 3 h and 6 h after PBMT. Muscle glycogen synthesis was measured 24 h after PBMT. This PBMT used a cluster of 69 LEDs (light-emitting diodes) with 35 red (630 ± 10 nm) and 34 infrared (850 ± 20 nm); 114 mW/cm for 90s (10 J/cm), 270 s (30 J/cm), 540 s (60 J/cm) applied on large muscle areas (back and hind legs) of the animals. The 10 J/cm group showed lower blood glucose levels and glucose variability over 6 h (5.92 mg/dL) compared to the sham (13.03 mg/dL), 30 J/cm (7.77 mg/dL) and 60 J/cm (9.07 mg/dL) groups. The PBMT groups had the greatest increase in muscle glycogen (10 J/cm > 60 J/cm > 30 J/cm > sham), characterizing a triphasic dose-response of PBMT. There was a strong negative correlation between blood glucose variability over 6 h and muscle glycogen concentration for 10 J/cm group (r = -0.94; p < .001) followed by 30 J/cm group (r = -0.84; p < .001) and 60 J/cm group(r = -0.73; p < .006). These results suggest that PBMT can play a very important role in the control of blood glucose levels, and its possible mechanism of action is the induction of greater muscle glycogen synthesis independently of physical exercise.
光生物调节疗法（PBMT）对肌肉骨骼组织的能量代谢有多种影响，例如增加糖原和三磷酸腺苷的合成。此外，这些影响可能归因于全身血糖控制。将 24 只 Wistar 大鼠随机平均分配到 4 组：假照射组、PBMT 10 J/cm、PBMT 30 J/cm 和 PBMT 60 J/cm。在照射前、照射后 1 小时、3 小时和 6 小时，动物禁食 6 小时以评估血糖。照射后 24 小时测量肌肉糖原合成。这种 PBMT 使用一组 69 个 LED（发光二极管），其中 35 个为红色（630±10nm），34 个为红外线（850±20nm）；114mW/cm，照射 90 秒（10 J/cm）、270 秒（30 J/cm）、540 秒（60 J/cm），照射于动物的大肌肉区域（背部和后腿）。与假照射组（13.03mg/dL）、30 J/cm 组（7.77mg/dL）和 60 J/cm 组（9.07mg/dL）相比，10 J/cm 组在 6 小时内血糖水平和血糖变异性更低（5.92mg/dL）。PBMT 组的肌肉糖原增加最大（10 J/cm＞60 J/cm＞30 J/cm＞假照射组），表现出 PBMT 的三相剂量反应。10 J/cm 组的血糖变异性与肌肉糖原浓度之间存在很强的负相关（r=-0.94；p<.001），其次是 30 J/cm 组（r=-0.84；p<.001）和 60 J/cm 组（r=-0.73；p<.006）。这些结果表明，PBMT 可以在血糖水平控制中发挥非常重要的作用，其可能的作用机制是独立于体育锻炼诱导更大的肌肉糖原合成。
