Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University, Guangzhou, China.
Guangdong Province Key Laboratory of Stomatology, Guangzhou, China.
J Cell Mol Med. 2020 May;24(10):5832-5841. doi: 10.1111/jcmm.15252. Epub 2020 Apr 16.
CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. However, the role of CA9 in the molecular mechanisms of tongue squamous cell carcinoma (TSCC) pathogenesis remains unclear. CA9 expression was analysed using the TCGA database, and its influence on survival was performed using Kaplan-Meier, LASSO and COX regression analyses. The correlation between CA9 and immune infiltration was investigated by CIBERSORT and ESTIMATE. Moreover, the relationship between CA9 expression and downstream molecular regulation pathways was analysed by GSEA, GO and WGCNA. CA9 expression correlated with clinical prognosis and tumour grade in TSCC. Moreover, CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. However, the follicular helper T cells, regulatory T cells, immune and stromal scores showed no significance between high and low CA9 expression groups. These findings suggested that CA9 plays a critical role of TSCC prognosis and tumour grade. CA9 expression significantly correlated with the regulation of cell differentiation, various oncogenes and cancer-associated pathways.
CA9 是碳酸酐酶家族的一员，通常在缺氧条件下在癌细胞中表达。然而，CA9 在舌鳞状细胞癌（TSCC）发病机制的分子机制中的作用尚不清楚。使用 TCGA 数据库分析 CA9 的表达，并使用 Kaplan-Meier、LASSO 和 COX 回归分析其对生存的影响。通过 CIBERSORT 和 ESTIMATE 研究 CA9 与免疫浸润的相关性。此外，通过 GSEA、GO 和 WGCNA 分析 CA9 表达与下游分子调节途径的关系。CA9 表达与 TSCC 的临床预后和肿瘤分级相关。此外，CA9 表达可能有助于调节癌细胞分化，并介导肿瘤相关基因和信号通路，包括细胞凋亡、缺氧、G2M 检查点、PI3K/AKR/mTOR 信号通路和 TGF-β信号通路。然而，高和低 CA9 表达组之间滤泡辅助 T 细胞、调节性 T 细胞、免疫和基质评分没有显着差异。这些发现表明 CA9 在 TSCC 的预后和肿瘤分级中起着关键作用。CA9 表达与细胞分化、各种癌基因和癌症相关途径的调节显著相关。
