Es-Soucratti Khadija, Hammoumi Abderrahman, Bouchrif Brahim, Asmai Rajaa, En-Nassiri Houda, Karraouan Bouchra
Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, Hassan II University of Casablanca.
Laboratory of Microbiology, Department of Food Hygiene, Products and Environment, Institut Pasteur du Maroc, Casablanca, Morocco.
Ital J Food Saf. 2020 Apr 6;9(1):8692. doi: 10.4081/ijfs.2020.8692. eCollection 2020 Mar 31.
Campylobacteriosis and spp resistance to antibiotics represents a serious worldwide public health problem thermophilic Campylobacters, in particular, are major causes of gastroenteritis in humans. The aim of this study was to determine the prevalence and antimicrobial resistance of isolated from chicken droppings, of commercial poultry in the city of Casablanca, Morocco. Between February and September 2017, 140 samples of chicken droppings were collected and analyzed by classical bacteriology methods for isolation and identification according to Moroccan Standard NM ISO/TS 10272-3 (2013), followed by molecular identification (PCR: polymerase chain reaction). Among the 140 samples, 102 (73%) were positive by spp. tests and 38 (27.14 %) were negative to spp. Among the positive colonies, 41 (40, 2%) were . Of the 41 isolates, resistance was detected to tetracycline (100%), erythromycin (97%), ampicillin (85%), ciprofloxacin (77%), amoxicillin/ clavulanic acid (61.4%), and gentamicin (12.0%). In conclusion, the data obtained in the current study demonstrate that the majority of isolates evaluated were resistant to antimicrobials of the cycline, macrolide, and fluoroquinolone families, and all of the isolates were susceptible to gentamicin. Fluoroquinolone is the drug of choice for treating infections. These results underline the need for prudent use of antibiotics in poultry production to minimize the spread of antibioticresistant spp.
弯曲杆菌病以及弯曲杆菌属对抗生素的耐药性是一个严重的全球性公共卫生问题。嗜热弯曲杆菌尤其是人类肠胃炎的主要病因。本研究的目的是确定从摩洛哥卡萨布兰卡市商业家禽的鸡粪中分离出的弯曲杆菌的流行情况和抗菌耐药性。2017年2月至9月期间，收集了140份鸡粪样本，并根据摩洛哥标准NM ISO/TS 10272-3（2013）采用经典细菌学方法进行分离和鉴定分析，随后进行分子鉴定（PCR：聚合酶链反应）。在140份样本中，102份（73%）弯曲杆菌属检测呈阳性，38份（27.14%）弯曲杆菌属检测呈阴性。在阳性菌落中，41份（40.2%）为空肠弯曲杆菌。在这41份空肠弯曲杆菌分离株中检测到对四环素（100%）、红霉素（97%）、氨苄西林（85%）、环丙沙星（77%）、阿莫西林/克拉维酸（61.4%）和庆大霉素（12.0%）耐药。总之，本研究获得的数据表明，所评估的大多数空肠弯曲杆菌分离株对四环素类、大环内酯类和氟喹诺酮类抗菌药物耐药，并且所有分离株对庆大霉素敏感。氟喹诺酮是治疗空肠弯曲杆菌感染的首选药物。这些结果强调了在家禽生产中谨慎使用抗生素以尽量减少抗生素耐药性空肠弯曲杆菌属传播的必要性。
