Department of Pathology and Public Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Department of Clinical Nutrition and Dietetics, College of Health Sciences, University of Sharjah, P. O. Box 27272, Sharjah, United Arab Emirates; Department of Nutrition and Food Technology, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Int J Food Microbiol. 2020 Aug 16;327:108656. doi: 10.1016/j.ijfoodmicro.2020.108656. Epub 2020 May 11.
Campylobacter is the world's leading cause of bacterial gastroenteritis, causing nearly 9 million cases of food poisoning in Europe every year. Poultry is considered the main source of Campylobacter infection to humans. The objectives of the study were to determine occurrence of C. jejuni and C. coli in chickens, the antimicrobial resistance, genotypes, and relatedness of the isolates. A total of 177 chicken samples obtained from informal butcher shops (fresh), formal poultry slaughterhouses (refrigerated) and retail market (frozen) were analyzed. Isolation of Campylobacter spp. was conducted according to the ISO 10272-2006 method. Multiplex PCR was used for confirmation and identification of the isolates. The disk diffusion method was used to determine the antimicrobial resistance of the isolates and multilocus sequence typing was used for genotyping. The proportion of samples with Campylobacter spp. was 31.6% among all chicken samples (fresh and refrigerated 47.5%, frozen 0%) C. coli was isolated from 42.4% of chicken samples obtained from butcher shops and from 18.6% of samples obtained in formal slaughterhouses. C. jejuni was isolated from 17.0% of samples obtained in butcher shops and formal slaughterhouses. Campylobacter spp. was not isolated in frozen chicken samples. All tested isolates showed resistance toward ciprofloxacin and susceptibility toward imipenem and all of the isolates were multidrug resistant toward 5 or more antimicrobials. Three sequence types were identified among 10 C. coli isolates and seven sequence types were identified among 10 C. jejuni isolates. Among sequence types, chicken isolates shared similarities of both phenotypic and genetic levels.
空肠弯曲菌是世界上导致细菌性肠胃炎的主要原因，每年在欧洲导致近 900 万例食物中毒。家禽被认为是人类弯曲菌感染的主要来源。本研究的目的是确定鸡中弯曲菌属的鸡空肠弯曲菌和大肠弯曲菌的感染情况、分离株的抗生素耐药性、基因型和相关性。本研究共分析了 177 份来自非正规肉铺（新鲜）、正规家禽屠宰场（冷藏）和零售市场（冷冻）的鸡肉样本。根据 ISO 10272-2006 方法进行弯曲菌属的分离。采用多重 PCR 进行分离株的确认和鉴定。采用纸片扩散法测定分离株的抗生素耐药性，采用多位点序列分型进行基因分型。所有鸡样本中弯曲菌属的比例为 31.6%（新鲜和冷藏样本为 47.5%，冷冻样本为 0%）。从肉铺获得的鸡样本中分离出 42.4%的大肠弯曲菌，从正规屠宰场获得的样本中分离出 18.6%的大肠弯曲菌。从肉铺和正规屠宰场获得的鸡样本中分离出 17.0%的空肠弯曲菌。冷冻鸡肉样本中未分离出弯曲菌属。所有测试的分离株对环丙沙星均表现出耐药性，对亚胺培南均表现出敏感性，对 5 种或更多种抗生素均表现出多药耐药性。在 10 株大肠弯曲菌分离株中鉴定出 3 种序列型，在 10 株空肠弯曲菌分离株中鉴定出 7 种序列型。在序列型中，鸡分离株在表型和遗传水平上具有相似性。
