Maeshima Shuya, Ida Yoshiyuki, Shimizu Ryo, Kawaji Yuki, Tamura Takashi, Nuta Junya, Hatamaru Keiichi, Itonaga Masahiro, Kudo Masatoshi, Kitano Masayuki
Second Department of Internal Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, Wakayama, 641-8509, Japan.
Department of Gastroenterology and Hepatology, Faculty of Medicine, Kindai University, 377-2 Ohno-higashi, Osaka-sayama, Osaka, 589-8511, Japan.
J Med Ultrason (2001). 2020 Jul;47(3):435-443. doi: 10.1007/s10396-020-01013-x. Epub 2020 Apr 16.
Animal studies of endoscopic ultrasound-guided radiofrequency ablation (EUS-RFA) of the liver have rarely been reported. We assessed the effectiveness and safety of EUS-RFA in pigs.
We conducted four experiments using newly designed RFA electrodes. In the first experiment, we ablated excised liver using 19 G electrodes with active electrode tips with lengths of 1, 1.5, and 2 cm. The second experiment was performed with the same electrodes as those used in the first experiment, but with the electrodes inserted into the livers of live pigs under EUS. In the third experiment, we tested the electrodes for water permeability. In the fourth experiment, we performed EUS-RFA on live pigs, using 19 G electrodes in 7/12 pigs and 18 G electrodes in 5/12 pigs. Complications were evaluated after 7 days of survival.
The newly designed RFA electrodes achieved ablation of the liver. In the first experiment, the maximal sizes of the ablation areas were 27, 26, 24, and 25 mm at 10, 20, 30, and 40 W, respectively, with the 2-cm electrode. In the second experiment, the maximal vertical sizes were 22, 23, 22, and 23 mm at 10, 20, 30, and 40 W, respectively, with the 2-cm electrode. In the third experiment, the 18 G electrode had better water permeability than the 19 G electrode. In the fourth experiment, all pigs survived. Complications occurred in 1/5 (18 G electrode) and 4/7 (19 G electrode) pigs.
We performed EUS-RFA in pigs and concluded that it may be feasible to perform RFA of lesions near the stomach.
关于肝脏的内镜超声引导下射频消融术（EUS-RFA）的动物研究鲜有报道。我们评估了EUS-RFA在猪身上的有效性和安全性。
我们使用新设计的射频消融电极进行了四项实验。在第一个实验中，我们使用19G电极，其活性电极尖端长度分别为1厘米、1.5厘米和2厘米，对切除的肝脏进行消融。第二个实验使用与第一个实验相同的电极，但在超声内镜引导下将电极插入活猪的肝脏。在第三个实验中，我们测试了电极的透水性。在第四个实验中，我们对活猪进行EUS-RFA，12头猪中有7头使用19G电极，5头使用18G电极。在存活7天后评估并发症情况。
新设计的射频消融电极实现了肝脏消融。在第一个实验中，使用2厘米电极时，在10瓦、20瓦、30瓦和40瓦功率下，消融区域的最大尺寸分别为27毫米、26毫米、24毫米和25毫米。在第二个实验中，使用2厘米电极时，在10瓦、20瓦、30瓦和40瓦功率下，最大垂直尺寸分别为22毫米、23毫米、22毫米和23毫米。在第三个实验中，18G电极的透水性比19G电极更好。在第四个实验中，所有猪均存活。1/5（18G电极组）和4/7（19G电极组）的猪出现了并发症。
我们在猪身上进行了EUS-RFA，并得出结论，对胃附近的病变进行射频消融可能是可行的。
