Zhu Yong, Shi Feng, Wang Meng, Ding Jian
Department of General Surgery, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820915958. doi: 10.1177/1533033820915958.
Rabs have been reported to be involved in the carcinogenesis process and in the progression of cancer. However, it is unclear whether or not Rab9 is associated with the development of cancer. In the present study, we aimed to investigate the role of Rab9 in the biological functions of gastric cancer cells. The gastric cancer cell lines AGS and MKN45 were transfected with siRNA-Rab9 to block the expression of Rab9. The cell viability, proliferation, migration, invasion, and apoptosis were examined using Cell Count Kit-8, colony formation, wound healing, Transwell, and flow cytometry assays, respectively. Our data showed that silencing of Rab9 significantly inhibited the viability, proliferation, migration, and invasion abilities of AGS and MKN45 cells. Moreover, transfection with siRab9 promoted the rate of apoptosis in AGS and MKN45 cells through regulating the Bcl-2-Bax axis and the Caspase cascade. We also found that silencing of Rab9 inhibited activation of the Akt signaling pathway by downregulating the phosphorylation level of Akt. In conclusion, our data suggest that Rab9 plays an oncogenic role in the progression of gastric cancer, providing a potential target for the treatment of gastric cancer.
据报道，Rabs参与了癌症发生过程以及癌症进展。然而，Rab9是否与癌症发展相关尚不清楚。在本研究中，我们旨在探究Rab9在胃癌细胞生物学功能中的作用。用siRNA-Rab9转染胃癌细胞系AGS和MKN45以阻断Rab9的表达。分别使用细胞计数试剂盒-8、集落形成、伤口愈合、Transwell和流式细胞术检测细胞活力、增殖、迁移、侵袭和凋亡。我们的数据表明，Rab9沉默显著抑制了AGS和MKN45细胞的活力、增殖、迁移和侵袭能力。此外，用siRab9转染通过调节Bcl-2-Bax轴和半胱天冬酶级联反应促进了AGS和MKN45细胞的凋亡率。我们还发现，Rab9沉默通过下调Akt的磷酸化水平抑制了Akt信号通路的激活。总之，我们的数据表明Rab9在胃癌进展中起致癌作用，为胃癌治疗提供了一个潜在靶点。
