用于球孢子菌酵母型-厚膜孢子型阶段最小抑菌浓度（MIC）检测的微量滴定法。
Microtiter method for MIC testing with spherule-endospore-phase Coccidioides immitis.
作者信息
Hector R F, Zimmer B L, Pappagianis D
机构信息
Cutter Biologicals, Berkeley, California 94701.
出版信息
J Clin Microbiol. 1988 Dec;26(12):2667-8. doi: 10.1128/jcm.26.12.2667-2668.1988.
Abstract
A method was developed for susceptibility testing with spherule-endospore-phase Coccidioides immitis by using a microtiter format. Isolated endospores were used to inoculate wells containing modified Converse medium with various concentrations of azole or nikkomycin antifungal substances which then were sealed with an acetate film. The plate was incubated at 37 degrees C with shaking for 96 h, after which the control wells had visible turbidity and endpoints were discernible. Microscopic examination revealed that both control and treatment wells maintained cells predominantly in the spherule-endospore phase of growth.
摘要
开发了一种使用微量滴定板形式对球囊-内生孢子期粗球孢子菌进行药敏试验的方法。使用分离出的内生孢子接种含有不同浓度唑类或多氧霉素抗真菌物质的改良康弗斯培养基的孔，然后用醋酸纤维素膜密封。将平板在37℃下振荡培养96小时，之后对照孔出现明显浑浊，可辨别终点。显微镜检查显示，对照孔和处理孔中的细胞主要维持在球囊-内生孢子生长阶段。
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本文引用的文献
1
NUTRITION OF THE PARASITIC PHASE OF COCCIDIOIDES IMMITIS IN A CHEMICALLY DEFINED LIQUID MEDIUM.粗球孢子菌寄生期在化学限定液体培养基中的营养研究
J Bacteriol. 1959 Aug;78(2):231-9. doi: 10.1128/jb.78.2.231-239.1959.
2
Immunity to coccidioi-domycosis induced in mice by purified spherule, arthrospore, and mycelial vaccines.纯化的球形体、关节孢子和菌丝体疫苗诱导小鼠对球孢子菌病产生的免疫力。
Trans N Y Acad Sci. 1960 Apr;22:436-49. doi: 10.1111/j.2164-0947.1960.tb00711.x.
3
Effect of surface active agents on endosporulation of Coccidioides immitis in a chemically defined medium.表面活性剂对在化学限定培养基中球孢子菌内孢子形成的影响。
J Bacteriol. 1957 Jul;74(1):106-7. doi: 10.1128/jb.74.1.106-107.1957.
4
Effect of physico-chemical environment of spherulation of Coccidioides immitis in a chemically defined medium.在化学成分明确的培养基中，粗球孢子菌形成球状体的物理化学环境的影响。
J Bacteriol. 1956 Dec;72(6):784-92. doi: 10.1128/jb.72.6.784-792.1956.
5
Growth of spherules of Coccidioides immitis in a chemically defined liquid medium.粗球孢子菌小球体在化学限定液体培养基中的生长
Proc Soc Exp Biol Med. 1955 Dec;90(3):709-11. doi: 10.3181/00379727-90-22144.
6
Inhibition of chitin synthesis in the cell wall of Coccidioides immitis by polyoxin D.多氧霉素D对粗球孢子菌细胞壁几丁质合成的抑制作用
J Bacteriol. 1983 Apr;154(1):488-98. doi: 10.1128/jb.154.1.488-498.1983.
7
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