Levine H B, Stevens D A, Cobb J M, Gebhardt A E
J Infect Dis. 1975 Oct;132(4):407-14. doi: 10.1093/infdis/132.4.407.
Administration of miconazole to mice infected with Coccidioides immitis prevented death in all cases; the infecting doses killed 60%-100% of the untreated animals. The drug's anticoccidioidal influence was also demonstrated by its capacity to limit fungal proliferation in the lungs. The endospore phase of C. immitis, which predominates in lesions, was more susceptible to miconazole than were the saprophytic arthrospore and mycelial phases. The drug was lethal to endospores in vitro in the presence or absence of human plasma, but plasma decelerated the rate of killing. A sensitive quantitative assay (using endospores) for the drug in plasma was developed, and the inefficacy of determining the sensitivity of strains with mycelia was demonstrated.
给感染粗球孢子菌的小鼠施用咪康唑可在所有情况下预防死亡；感染剂量可导致60%-100%未治疗动物死亡。该药物的抗球孢子菌作用还通过其限制肺部真菌增殖的能力得以体现。在病变中占主导的粗球孢子菌内生孢子期比腐生关节孢子期和菌丝体期对咪康唑更敏感。无论有无人血浆存在，该药物在体外对内生孢子均具有致死性，但血浆会减缓杀灭速度。开发了一种用于检测血浆中该药物的灵敏定量测定法（使用内生孢子），并证明了用菌丝体测定菌株敏感性的无效性。
