Student Research Committee, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Photobiomodul Photomed Laser Surg. 2020 Apr;38(4):222-231. doi: 10.1089/photob.2019.4773.
Enhanced sperm motility is necessary for the successful journey of sperm inside the female genital tract, successful fertilization, and the increased chance of pregnancy. We investigated the impact of red and near-infrared (NIR) ranges of photobiomodulation (PBM) alone and together on fresh human sperm to validate an optimized PBM protocol that would maximize sperm motility and viability . We randomly divided 30 normal human semen samples into 3 different PBM protocols (red, NIR, and red+NIR lasers). Each sample was divided into four subparts, one control group sample and three experimental group samples. Each experimental group received one of the PBM protocols (red, NIR, or red+NIR). Each protocol was adjusted to three energy densities (0.6, 1.2, and 2.4 J/cm). After exposure to the selected protocol, we determined the percentage of either viable or progressive sperm motility (PSM) and measured the DNA Fragmentation Index (DFI). The NIR and red+NIR lasers at 2.4 J/cm energy density significantly increased PSM after 60 min compared with the control groups [least significant difference (LSD) test, = 0.023 and = 0.04, respectively]. Samples treated with the red laser at 0.6 J/cm had significantly decreased viability compared with the control group (LSD test, = 0.003). Samples treated with the red+NIR lasers had significantly decreased viability at 0.6 J/cm ( = 0.003), 1.2 J/cm ( = 0.001), and 2.4 J/cm ( = 0.04) energy densities when compared with the control groups. The NIR laser resulted in no significant difference in sperm viability between the control and experimental groups. At 120 min after exposure, treatment with the red+NIR and red lasers at 2.4 J/cm density significantly increased DFI compared to the control groups (LSD test, = 0.000, = 0.007). In this study, sperm motility, viability, and DFI data confirmed the superiority of the NIR laser at 0.6 J/cm energy density compared with the red and red+NIR PBM protocols.
提高精子的活动力对于精子在女性生殖道内的成功游动、成功受精以及增加怀孕机会都是必要的。我们研究了单独和联合使用红光和近红外（NIR）范围的光生物调节（PBM）对新鲜人类精子的影响，以验证一个优化的 PBM 方案，该方案将最大限度地提高精子活力和活力。我们将 30 个正常的人类精液样本随机分为 3 个不同的 PBM 方案（红光、NIR 和红光+NIR 激光）。每个样本分为 4 个亚部分，一个对照组样本和 3 个实验组样本。每个实验组接受一个 PBM 方案（红光、NIR 或红光+NIR）。每个方案都调整到三个能量密度（0.6、1.2 和 2.4 J/cm）。在暴露于所选方案后，我们确定了有活力或进行性精子运动（PSM）的百分比，并测量了 DNA 片段化指数（DFI）。与对照组相比，NIR 和红光+NIR 激光在 2.4 J/cm 能量密度下 60 分钟后显著增加了 PSM[最小显著差异（LSD）检验，分别为 = 0.023 和 = 0.04]。与对照组相比，用 0.6 J/cm 红光处理的样本活力显著降低（LSD 检验， = 0.003）。用红光+NIR 激光处理的样本在 0.6 J/cm（ = 0.003）、1.2 J/cm（ = 0.001）和 2.4 J/cm（ = 0.04）能量密度时，与对照组相比，活力显著降低。NIR 激光在对照组和实验组之间的精子活力没有显著差异。暴露后 120 分钟，用红光+NIR 和红光激光在 2.4 J/cm 密度下处理显著增加了 DFI，与对照组相比（LSD 检验， = 0.000， = 0.007）。在这项研究中，精子运动、活力和 DFI 数据证实了 NIR 激光在 0.6 J/cm 能量密度下比红光和红光+NIR PBM 方案更优越。
