Institute of Urban and Rural Mining, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China.
National-Local Joint Engineering Research Center for Biomass Refining and High-Quality Utilization, Changzhou University, No. 21 Gehu Road, Wujin District, Changzhou 213164, China.
FEMS Microbiol Lett. 2020 May 1;367(9). doi: 10.1093/femsle/fnaa067.
Oils in food waste can pollute the environment and negatively affect human health. Biodegradation is a promising method for disposing of waste edible oils. In this study, an oil-degrading bacterium was isolated from kitchen waste for efficient degradation of edible oils. Its growth and oil degradation characteristics were investigated in basic salt medium with edible oils as the sole carbon and energy source; the triacylglycerol lipase gene (EC 3.1.1.3) was cloned and expressed in Escherichia coli. A novel oil-degrading bacterium assigned as IUMR B67 was successfully isolated. Morphological and molecular analyses revealed that strain IUMR B67 belongs to Kosakonia cowanii. After 144 h of incubation, the oil degradation rate at 37°C was 95.80%. Optimal conditions for IUMR B67 were recorded at 37°C and 0.1% NaCl with 0.1% ammonium sulfate supplementation. The lipase gene of strain IUMR B67 was determined to be 912 base pairs, and the lipase activity of the expressed protein was 3.02 U/mL, which was significantly higher than the control (P < 0.05). Overall, Kosakonia cowanii IUMR B67 is a novel edible oil-degrading strain that can hydrolyze oil via its lipase activity, which may be useful in the disposal of oils and oily food waste.
厨余废油会污染环境并对人类健康产生负面影响。生物降解是处理废弃食用油的一种很有前途的方法。在这项研究中，从厨房垃圾中分离出了一种能够高效降解食用油的降油细菌。在以食用油为唯一碳源和能源的基础盐培养基中，研究了其生长和油降解特性；克隆并在大肠杆菌中表达了三酰基甘油脂肪酶基因（EC 3.1.1.3）。成功分离出一种新型的油降解细菌，命名为IUMR B67。形态学和分子分析表明，菌株 IUMR B67 属于考克氏菌属。在 37°C 下孵育 144 小时后，油的降解率为 95.80%。IUMR B67 的最佳条件为 37°C 和 0.1%NaCl，并添加 0.1%硫酸铵。确定菌株 IUMR B67 的脂肪酶基因长 912 个碱基对，表达蛋白的脂肪酶活性为 3.02 U/mL，明显高于对照组（P<0.05）。总的来说，考克氏菌属 IUMR B67 是一种新型的食用油降解菌株，它可以通过其脂肪酶活性水解油脂，这在处理油脂和含油食物废物方面可能很有用。
