LM-UGent, Laboratory of Microbiology, Faculty of Sciences, Ghent University, Ghent, Belgium.
Syst Appl Microbiol. 2013 Jul;36(5):309-19. doi: 10.1016/j.syapm.2013.03.005. Epub 2013 Apr 28.
The taxonomy of Enterobacter has a complicated history, with several species transferred to and from this genus. Classification of strains is difficult owing to its polyphyletic nature, based on 16S rRNA gene sequences. It has been previously acknowledged that Enterobacter contains species which should be transferred to other genera. In an attempt to resolve the taxonomy of Enterobacter, MLSA based on partial sequencing of protein-encoding genes (gyrB, rpoB, infB and atpD) was performed on the type strains and reference strains of Enterobacter, Cronobacter and Serratia species, as well as members of the closely related genera Citrobacter, Klebsiella, Kluyvera, Leclercia, Mangrovibacter, Raoultella and Yokenella. Phylogenetic analyses of the concatenated nucleotide sequences revealed that Enterobacter can be divided into five strongly supported MLSA groups, suggesting that the species should be reclassified into five different genera. Further support for this was provided by a concatenated amino acid tree, phenotypic characteristics and fatty acid profiles, enabling differentiation of the MLSA groups. Three novel genera are proposed: Lelliottia gen. nov., Pluralibacter gen. nov. and Kosakonia gen. nov. and the following new combinations: Lelliottia nimipressuralis comb. nov., Lelliottia amnigena comb. nov., Pluralibacter gergoviae comb. nov., Pluralibacter pyrinus comb. nov., Kosakonia cowanii comb. nov., Kosakonia radicincitans comb. nov., Kosakonia oryzae comb. nov., Kosakonia arachidis comb. nov., Cronobacter helveticus comb. nov. and Cronobacter pulveris comb. nov. Additionally, the novel epithet Cronobacter zurichensis nom. nov. is proposed for the reclassification of Enterobacter turicensis into the genus Cronobacter, as Cronobacter turicensis (Iversen et al., 2008) is already in use.
肠杆菌的分类学历史复杂，有几个种被转移到这个属和从这个属转移出去。由于其多系性，基于 16S rRNA 基因序列，对菌株进行分类很困难。先前已经认识到肠杆菌包含应该转移到其他属的种。为了尝试解决肠杆菌的分类学问题，对肠杆菌、克氏杆菌和沙雷氏菌属的模式株和参考株，以及近缘属柠檬酸杆菌、克雷伯氏菌、克吕沃尔氏菌、莱氏杆菌、红树林杆菌、罗阿氏菌和叶杆菌的部分蛋白编码基因（gyrB、rpoB、infB 和 atpD）进行了基于 MLSA 的分类。基因序列的系统发育分析显示，肠杆菌可以分为五个强烈支持的 MLSA 组，表明这些种应该重新分类为五个不同的属。这一结论得到了氨基酸串联树、表型特征和脂肪酸图谱的进一步支持，使 MLSA 组得以区分。提出了三个新属：Lelliottia 属、Pluralibacter 属和 Kosakonia 属，并提出了以下新组合：Lelliottia nimipressuralis 组合、Lelliottia amnigena 组合、Pluralibacter gergoviae 组合、Pluralibacter pyrinus 组合、Kosakonia cowanii 组合、Kosakonia radicincitans 组合、Kosakonia oryzae 组合、Kosakonia arachidis 组合、Cronobacter helveticus 组合和 Cronobacter pulveris 组合。此外，还提出了 Cronobacter zurichensis nom. nov. 的新学名，用于将肠杆菌 turicensis 重新分类为 Cronobacter 属，因为 Cronobacter turicensis（Iversen 等人，2008）已经在使用。
