Foodborne Toxin Detection and Prevention Research Unit, Western Regional Research Center, United States Department of Agriculture, Agricultural Research Service, Albany, California, United States of America.
Plant and Microbial Biology Department, University of California, Berkeley, California, United States of America.
PLoS One. 2020 Apr 17;15(4):e0231781. doi: 10.1371/journal.pone.0231781. eCollection 2020.
The mushroom poison that causes the most deaths is the class of toxins known as amatoxins. Current methods to sensitively and selectively detect these toxins are limited by the need for expensive equipment, or they lack accuracy due to cross-reactivity with other chemicals found in mushrooms. In this work, we report the development of a competition-based lateral flow immunoassay (LFIA) for the rapid, portable, selective, and sensitive detection of amatoxins. Our assay clearly indicates the presence of 10 ng/mL of α-AMA or γ-AMA and the method including extraction and detection can be completed in approximately 10 minutes. The test can be easily read by eye and has a presumed shelf-life of at least 1 year. From testing 110 wild mushrooms, the LFIA identified 6 out of 6 species that were known to contain amatoxins. Other poisonous mushrooms known not to contain amatoxins tested negative by LFIA. This LFIA can be used to quickly identify amatoxin-containing mushrooms.
导致死亡人数最多的毒蕈是一类被称为鹅膏蕈碱的毒素。目前，用于灵敏和选择性检测这些毒素的方法受到需要昂贵设备的限制，或者由于与蘑菇中其他化学物质的交叉反应而缺乏准确性。在这项工作中，我们报告了一种基于竞争的侧向流动免疫分析（LFIA）的发展，用于快速、便携、选择性和灵敏地检测鹅膏蕈碱。我们的测定法清楚地表明存在 10ng/mL 的 α-AMA 或 γ-AMA，并且包括提取和检测在内的方法可以在大约 10 分钟内完成。该测试可以通过肉眼轻松读取，保质期至少为 1 年。通过测试 110 种野生蘑菇，LFIA 鉴定出 6 种已知含有鹅膏蕈碱的蘑菇。其他已知不含鹅膏蕈碱的有毒蘑菇通过 LFIA 测试呈阴性。这种 LFIA 可用于快速识别含有鹅膏蕈碱的蘑菇。
