State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Haidian District, P. O. Box 2871, Beijing, 100085, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Environ Sci Pollut Res Int. 2020 Jun;27(17):22120-22126. doi: 10.1007/s11356-020-08770-9. Epub 2020 Apr 17.
This study was performed to explore acute toxicity of biologically synthesized gold nanoparticles (AuNPs) to a model organism Daphnia magna. Hence, using aqueous extract of marine macroalga Saccharina japonica, two AuNPs including SJ-AuNPs-72 (72.6 ± 43.8 nm) and SJ-AuNPs-10 (10.8 ± 2.8 nm) were synthesized. These AuNPs were characterized by different techniques such as UV-Vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and energy-dispersive X-ray spectroscopy (EDX). The 48-h LC values of SJ-AuNPs-72 and SJ-AuNPs-10 to D. magna were 1.57 ± 0.07 and 2.69 ± 0.12 mg/L, respectively, showing greater toxicity of SJ-AuNPs-72. After exposure of daphnids to treatments, AuNPs were accumulated in gut tract, and lipid droplets under the Daphnia carapace were also observed. Whereas studies on toxicity of biosynthesized AuNPs are still scarce, the achievements of this work are helpful for understanding the toxicity of biosynthesized AuNPs to crustacean D. magna.
本研究旨在探索生物合成的金纳米粒子（AuNPs）对模式生物大型溞（Daphnia magna）的急性毒性。因此，使用海洋大型海藻裙带菜（Saccharina japonica）的水提物，合成了两种 AuNPs，包括 SJ-AuNPs-72（72.6±43.8nm）和 SJ-AuNPs-10（10.8±2.8nm）。这些 AuNPs 通过不同的技术进行了表征，如紫外-可见分光光度法、透射电子显微镜（TEM）、动态光散射（DLS）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和能谱（EDX）。SJ-AuNPs-72 和 SJ-AuNPs-10 对大型溞的 48 小时 LC 值分别为 1.57±0.07 和 2.69±0.12mg/L，表明 SJ-AuNPs-72 的毒性更大。在暴露于处理后，AuNPs 被积累在肠道中，在大型溞的甲壳下也观察到了脂质滴。虽然关于生物合成的 AuNPs 的毒性研究仍然很少，但这项工作的成果有助于理解生物合成的 AuNPs 对甲壳类动物大型溞的毒性。
