Center for Transdisciplinary Research (CFTR), Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Saveetha University, Chennai, India.
Department of Chemistry, Maulana Azad National Institute of Technology, Bhopal, India.
Biomed Res Int. 2022 Jul 13;2022:5746761. doi: 10.1155/2022/5746761. eCollection 2022.
A major paradigm shift in the field of nanobiotechnology is the invention of an eco-friendly, economical, and green approach for synthesis of metal nanoparticles. In the present study, we have synthesized gold nanoparticles (AuNPs) using aqueous extracts of marine brown seaweed Sargassum longifolium. The synthesized nanoparticle was subjected to characterization using different techniques such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy, atomic force microscope, scanning electron microscope, transmission electron microscope, and elemental dispersive X-ray diffraction. Further, the seaweed extract and the synthesized AuNPs were evaluated for its anticancer effect using MG-63 human osteosarcoma cells besides in vitro antioxidant effect. The formation of S. longifolium-mediated synthesis of gold nanoparticles was demonstrated by UV-Vis spectroscopy. Presence of elemental gold was confirmed by EDX analysis. TEM analysis demonstrated spherical morphology of the synthesized AuNPs and SEM analysis revealed the particle size to be in the range of 10-60 nm. The FTIR showed the presence of hydroxyl functional groups. The toxicity of S. longifolium extract and the synthesized AuNPs was tested using brine shrimp lethality assay at different concentrations with results showing both seaweed extract and AuNPs to be nontoxic. Both S. longifolium and AuNPs exhibited significant antioxidant activity by scavenging DPPH free radicals and HO radicals. Significant antiproliferative effect was observed against MG-63 osteosarcoma cells. It was also shown that the seaweed extract and the AuNPs induced cytotoxicity in cell lines by mechanism of apoptosis. In conclusion, this study provided insight on AuNPs synthesized from S. longifolium as a potent antioxidant and anticancer agent.
海洋褐藻 Sargassum longifolium 水提物介导合成金纳米粒子及其生物活性评价
纳米生物技术领域的一个主要范式转变是发明了一种环保、经济和绿色的方法来合成金属纳米粒子。在本研究中，我们使用海洋褐藻 Sargassum longifolium 的水提物合成了金纳米粒子（AuNPs）。使用不同的技术对合成的纳米粒子进行了表征，如紫外可见光谱、傅里叶变换红外光谱、原子力显微镜、扫描电子显微镜、透射电子显微镜和元素分散 X 射线衍射。此外，还评估了褐藻提取物和合成的 AuNPs 在体外抗氧化作用之外对 MG-63 人骨肉瘤细胞的抗癌作用。通过紫外可见光谱证明了 S. longifolium 介导的金纳米粒子的形成。通过 EDX 分析证实了元素金的存在。TEM 分析表明合成的 AuNPs 呈球形形态，SEM 分析表明粒径在 10-60nm 范围内。FTIR 显示存在羟基官能团。使用不同浓度的盐水虾致死试验测试了 S. longifolium 提取物和合成的 AuNPs 的毒性，结果表明，褐藻提取物和 AuNPs 均无毒性。S. longifolium 和 AuNPs 均通过清除 DPPH 自由基和 HO 自由基表现出显著的抗氧化活性。对 MG-63 骨肉瘤细胞表现出显著的增殖抑制作用。还表明，褐藻提取物和 AuNPs 通过细胞凋亡机制诱导细胞系的细胞毒性。总之，本研究为从 S. longifolium 合成的 AuNPs 作为一种有效的抗氧化和抗癌剂提供了新的认识。
