State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, Inner Mongolia, China.
Environ Sci Pollut Res Int. 2020 Jul;27(19):24081-24089. doi: 10.1007/s11356-020-08555-0. Epub 2020 Apr 17.
Fipronil is an insecticide commonly used in agriculture. We report here on the sublethal and sub-chronic effects of fipronil on non-target topmouth gudgeon (Pseudorasbora parva) at environmentally relevant levels. The results showed that fipronil did not cause significant changes in brain acetylcholinesterase activities, glutathione S-transferase (GST) activities in the intestine, and GST, glutamic pyruvic transaminase (GPT), and glutamic oxaloacetic transaminase (GOT) activities in the liver tissues at environmentally relevant levels for 96-h exposure. In the further test for a 12-day exposure, dose-dependent responses of the serum GPT and GOT activities were observed in all treated groups with sublethal concentrations of fipronil. Furthermore, fipronil could reduce the liver mitochondrial membrane fluidity of P. parva, especially with high concentration of fipronil at high temperature. The results suggest that serum GPT and GOT in P. parva might be useful biomarkers for effects of fipronil exposure at environmentally relevant level, and reducing fluidity of liver mitochondrial membrane may be one toxic mechanism of fipronil.
氟虫腈是一种常用于农业的杀虫剂。我们在此报告了在环境相关水平下，氟虫腈对非靶标麦穗鱼（Pseudorasbora parva）的亚致死和亚慢性影响。结果表明，在 96 小时的暴露时间内，氟虫腈在环境相关水平下不会导致鱼脑组织乙酰胆碱酯酶活性、肠道谷胱甘肽 S-转移酶（GST）活性以及肝组织 GST、谷丙转氨酶（GPT）和谷草转氨酶（GOT）活性发生显著变化。在进一步的 12 天暴露试验中，所有用亚致死浓度氟虫腈处理的鱼血清 GPT 和 GOT 活性均呈现出剂量依赖性反应。此外，氟虫腈可降低 P. parva 的肝线粒体膜流动性，尤其是在高温和高浓度氟虫腈条件下。结果表明，血清 GPT 和 GOT 可能是评估环境相关水平下氟虫腈暴露影响的有用生物标志物，而肝线粒体膜流动性的降低可能是氟虫腈的一种毒性机制。
