Institute of Mass Spectrometry and Atmospheric Environment, and Guangdong Provincial Engineering Research Centre for On-line Source Apportionment System of Air Pollution, Jinan University, Guangzhou, China.
Phytochem Anal. 2020 Nov;31(6):739-746. doi: 10.1002/pca.2937. Epub 2020 Apr 18.
Vinca alkaloids are important sources for producing anticancer drugs from Catharanthus roseus. The phosphorus of soil is one of crucial factors for planting C. roseus.
We aim to develop an in vivo sampling technique coupled with direct mass spectrometry with wooden tip for investigating distributions and changes of alkaloids in flowers, leaves, stems, veins and roots of living C. roseus under low-phosphorus stress.
Living C. roseus were prepared under low-phosphorus stress (n = 10) and control conditions (n = 10). Wooden-tip electrospray ionisation mass spectrometry and conventional liquid chromatography-mass spectrometry were applied to analyse living C. roseus and extracts of C. roseus, respectively.
Distributions and changes of serpentine, vindoline, catharanthine, and anhydrovinblastine in living C. roseus under low-phosphorus stress and control conditions were successfully obtained.
Compared to control soil conditions, low-phosphorus soil was found to induce C. roseus to generate more serpentine but less catharanthine and vindoline in leaves, veins, stems and roots, and to generate more anhydrovinblastine in flowers, leaves, stems and roots. Overall, our results showed a simple, rapid, and effective method for in vivo sampling and direct analysis of living plants.
长春花生物碱是从长春花中提取抗癌药物的重要来源。土壤中的磷是种植长春花的关键因素之一。
我们旨在开发一种体内采样技术，结合木制尖端的直接质谱法，用于研究在低磷胁迫下活体长春花中生物碱在花、叶、茎、脉和根中的分布和变化。
在低磷胁迫下（n=10）和对照条件下（n=10）制备活体长春花。分别采用木制尖端电喷雾离子化质谱法和常规液相色谱-质谱法分析活体长春花和长春花提取物。
成功获得了低磷胁迫和对照条件下活体长春花中蛇根碱、长春碱、长春质碱和脱水长春碱的分布和变化。
与对照土壤条件相比，低磷土壤被发现诱导长春花在叶、脉、茎和根中产生更多的蛇根碱，而产生更少的长春质碱和长春碱，并在花、叶、茎和根中产生更多的脱水长春碱。总的来说，我们的结果表明了一种简单、快速和有效的活体植物体内采样和直接分析方法。
