Centro Nacional de Secuenciación Genómica - CNSG, Universidad de Antioquia, Medellín, Antioquia, Colombia.
Empresas Públicas de Medellín-EPM, Medellín, Antioquia, Colombia.
Sci Total Environ. 2020 Jul 15;726:138479. doi: 10.1016/j.scitotenv.2020.138479. Epub 2020 Apr 11.
Anaerobic digestion is a microbe-driven process widely applied to treat activated sludge from municipal wastewater treatment plants. It is one of the most efficient solutions for sludge reduction along with biogas production. However, the knowledge of the microbial consortium involved in this process is still unknown in full-scale anaerobic digesters from Latin America. This study aimed to elucidate the dynamics of the microbial community of a full-scale anaerobic digester for a year using 16S rDNA amplicon sequencing with the Illumina Miseq platform. The results showed fluctuations in the frequencies of dominant phyla with a decrease of Proteobacteria and Bacteroidetes after a temporary suspension of anaerobic digester. The core community was affiliated with bacterial phyla Firmicutes, Actinobacteria, Proteobacteria, and Chloroflexi. The core community was represented by 154 OTUs that accounted for 74% of all the processed reads. The Anaerolineaceae family, within Chloroflexi phylum, was the most frequently observed taxonomic group in all samples analyzed. Despite the microbial fluctuations, the biogas production was stable over the studied year (average 66% methane production), which might indicate a functional redundancy in the microbial consortium.
厌氧消化是一种微生物驱动的过程，广泛应用于处理城市污水处理厂的活性污泥。它是减少污泥和生产沼气的最有效方法之一。然而，在拉丁美洲的大型厌氧消化器中，对于涉及的微生物群落的了解还不完全。本研究旨在使用 Illumina Miseq 平台的 16S rDNA 扩增子测序，阐明大型厌氧消化器中微生物群落的动态变化，为期一年。结果表明，随着厌氧消化器的暂时停止，优势门的频率出现波动，变形菌门和拟杆菌门的丰度下降。核心群落与厚壁菌门、放线菌门、变形菌门和绿弯菌门等细菌门有关。核心群落由占所有处理读长 74%的 154 个 OTUs 组成。绿弯菌门中的 Anaerolineaceae 科是所有分析样本中最常观察到的分类群。尽管微生物发生波动，但沼气产量在研究期间保持稳定（平均甲烷产量为 66%），这可能表明微生物群落具有功能冗余性。
