International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi, China; Key Laboratory of Environmental Engineering, Shaanxi, China; Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, China.
International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi, China; Key Laboratory of Environmental Engineering, Shaanxi, China; Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, China.
Bioresour Technol. 2020 Dec;317:123994. doi: 10.1016/j.biortech.2020.123994. Epub 2020 Aug 11.
The effects of long-term acclimatization on the optimum food waste to cow manure ratio (FW/CM) and substrate to inoculum ratio (S/I) in anaerobic codigestion with FW and CM were investigated by batch trials. For the unacclimated sludge, the highest CH yields of 646.6 and 653.4 mL/g VS were achieved under the optimum FW/CM (2.5 VS/VS) and S/I (0.07 VS/VS) ratios, respectively. After more than 550 days of acclimatization, the optimum FW/CM and S/I of the acclimated sludge were improved to 3.4 and 0.68 VS/VS with more anaerobic digestion enzymes and lignocellulose, respectively. Based on high-throughput sequencing analysis, the microbial community structures of bacteria, fungi, and archaea were changed, which was the main reason for the change in the optimum FW/CM and S/I. Therefore, the FW/CM and S/I should be periodically optimized during the long-term operation of codigestion to improve the codigestion efficiency for biogas production.
采用批式试验研究了长期驯化对厨余垃圾(FW)与牛粪(CM)共厌氧消化中最佳厨余垃圾与牛粪比例(FW/CM)和基质与接种物比例(S/I)的影响。对于未驯化的污泥,在最佳 FW/CM(2.5 VS/VS)和 S/I(0.07 VS/VS)比下,分别获得了 646.6 和 653.4 mL/g VS 的最高 CH 产率。经过 550 多天的驯化,驯化污泥的最佳 FW/CM 和 S/I 分别提高到 3.4 和 0.68 VS/VS,这是由于分别增加了更多的厌氧消化酶和木质纤维素。基于高通量测序分析,细菌、真菌和古菌的微生物群落结构发生了变化,这是 FW/CM 和 S/I 变化的主要原因。因此,在共消化的长期运行过程中,应定期优化 FW/CM 和 S/I,以提高沼气生产的共消化效率。