Chen Fei, Li Yuancheng, Qin Na, Wang Fengliang, Du Jiangbo, Wang Cheng, Du Fangzhi, Jiang Tao, Jiang Yue, Dai Juncheng, Hu Zhibin, Lu Cheng, Shen Hongbing
Department of Breast Surgery, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, Jiangsu 210004, China;Department of Epidemiology, Center for Global Health, School of Public Health.
Department of Epidemiology, Center for Global Health, School of Public Health.
J Biomed Res. 2020 Jan 29;34(2):129-138. doi: 10.7555/JBR.34.20190111.
Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer that currently lacks effective biomarkers and therapeutic targets required to investigate the diagnosis and treatment of TNBC. Here we performed a comprehensive differential analysis of 165 TNBC samples by integrating RNA-seq data of breast tumor tissues and adjacent normal tissues from both our cohort and The Cancer Genome Atlas (TCGA). Pathway enrichment analysis was conducted to evaluate the biological function of TNBC-specific expressed genes. Further multivariate Cox proportional hazard regression was performed to evaluate the effect of these genes on TNBC prognosis. In this report, we identified a total of 148 TNBC-specific expressed genes that were primarily enriched in mammary gland morphogenesis and hormone levels related pathways, suggesting that mammary gland morphogenesis might play a unique role in TNBC patients differing from other breast cancer types. Further survival analysis revealed that nine genes ( , , , , , , , , and ) were significantly associated with the prognosis of TNBC patients, while three of them ( , , and ) were involved in the hormone-related pathways. These findings indicated the vital role of the hormone-related genes in TNBC tumorigenesis and may provide some independent prognostic markers as well as novel therapeutic targets for TNBC.
三阴性乳腺癌（TNBC）是一种侵袭性乳腺癌亚型，目前缺乏用于研究TNBC诊断和治疗所需的有效生物标志物和治疗靶点。在此，我们通过整合来自我们的队列以及癌症基因组图谱（TCGA）的乳腺肿瘤组织和相邻正常组织的RNA测序数据，对165例TNBC样本进行了全面的差异分析。进行通路富集分析以评估TNBC特异性表达基因的生物学功能。进一步进行多变量Cox比例风险回归以评估这些基因对TNBC预后的影响。在本报告中，我们共鉴定出148个TNBC特异性表达基因，这些基因主要富集于乳腺形态发生和激素水平相关通路，这表明乳腺形态发生可能在TNBC患者中发挥与其他乳腺癌类型不同的独特作用。进一步的生存分析显示，九个基因（ ， ， ， ， ， ， ， ，和 ）与TNBC患者的预后显著相关，其中三个基因（ ， ，和 ）参与激素相关通路。这些发现表明激素相关基因在TNBC肿瘤发生中起着至关重要的作用，并可能为TNBC提供一些独立的预后标志物以及新的治疗靶点。
