Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.
Industrial Insect Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.
Antonie Van Leeuwenhoek. 2020 Jul;113(7):1009-1021. doi: 10.1007/s10482-020-01413-6. Epub 2020 Apr 18.
Two bacterial strains, designated 2DFWM-2 and FW10M-9, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea. 16S rRNA and rpoB gene sequences showed that strain 2DFWM-2 formed a separate branch with Lactococcus allomyrinae 1JSPR-7 in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies. ANI and dDDH values between 2DFWM-2 and Lactococcus allomyrinae 1JSPR-7 were 93.30% and 53.20%, respectively. Based on the 16S rRNA gene sequence analysis, strain FW10M-9 was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species. The ANI values of strain FW10M-9 with the closely species Xylanimonas pachnodae NBRC 107786, Xylanimonas allomyrinae 2JSPR-7, Isoptericola variabilis JCM 11754 and Xylanimonas cellulosilytica DSM 15894 was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively. On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2 represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp. nov. is proposed, and the type strain is 2DFWM-2 (= KACC 19320 = NBRC 113069). And, strain FW10M-9 represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp. nov. is proposed, and the type strain is FW10M-9 (= KACC 19331 = NBRC 113053).
两株细菌菌株，命名为 2DFWM-2 和 FW10M-9，是从韩国完州郡国立农业科学院饲养的 Protaetia brevitarsis seulensis 幼虫肠道中分离出来的。16S rRNA 和 rpoB 基因序列表明，菌株 2DFWM-2 与乳球菌属的 Lactococcus allomyrinae 1JSPR-7 形成一个单独的分支，与乳球菌属 Lactococcus lactis 亚种相邻。2DFWM-2 与 Lactococcus allomyrinae 1JSPR-7 之间的 ANI 和 dDDH 值分别为 93.30%和 53.20%。根据 16S rRNA 基因序列分析，FW10M-9 菌株被分类为 Xylanimonas 属，与 Xylanimonas 种的序列相似度为 96.9-98.5%。FW10M-9 菌株与近缘种 Xylanimonas pachnodae NBRC 107786、Xylanimonas allomyrinae 2JSPR-7、Isoptericola variabilis JCM 11754 和 Xylanimonas cellulosilytica DSM 15894 的 ANI 值分别为 81.5%、81.2%、81.0%和 84.1%，由 GGDC 估计的 dDDH 值分别为 24.3%、24.3%、29.3%和 28.1%。基于表型和基因型数据，建议 2DFWM-2 菌株代表乳球菌属的一个新种，命名为 Lactococcus protaetiae sp. nov.，其模式菌株为 2DFWM-2(=KACC 19320=NBRC 113069)。此外，FW10M-9 菌株代表 Xylanimonas 属的一个新种，命名为 Xylanimonas protaetiae sp. nov.，其模式菌株为 FW10M-9(=KACC 19331=NBRC 113053)。
