Rodríguez-Morales Olivia, Cabrera-Mata Juan José, Carrillo-Sánchez Silvia Del C, Gutiérrez-Ocejo Rodolfo A, Baylón-Pacheco Lidia, Pérez-Reyes Olga L, Rosales-Encina José Luis, Aranda-Fraustro Alberto, Hernández-García Sergio, Arce-Fonseca Minerva
Laboratory of Molecular Immunology and Proteomics, Department of Molecular Biology, National Institute of Cardiology "Ignacio Chávez", Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City 14080, Mexico.
Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute, Av. Instituto Politécnico Nacional No. 2508, Col. San Pedro Zacatenco, Gustavo A. Madero, Mexico City 07360, Mexico.
Pathogens. 2020 Nov 23;9(11):974. doi: 10.3390/pathogens9110974.
Chagas disease is a major public health problem in Latin America. The mixed Th1/Th2 immune response is required against . Electrolyzed oxidizing water (EOW) has been shown to have germicidal efficacy. The objective of this study was to evaluate the EOW effectiveness in infected BALB/c mice clinically, immunologically, and histologically. The severity of the infection was assessed by parasitaemia, general health condition, mortality, mega syndromes, and histological lesions. IgG, TNF-alpha, IFN-gamma, and IL-1 beta levels were quantified. The EOW administration showed a beneficial effect on parasitaemia, general physical condition, and mortality. High levels of IgG1 at 50 days postinfection were observed. Prophylactic EOW treatment was able to induce a predominantly TH1 immune response based on an IgG2a levels increase at the late acute phase, and a 10-fold increase of INF-gamma in whole acute phase. EOW was able to control the acute phase infection as effectively as benznidazole. Splenomegaly was caused by EOW treatment and lymphadenopathy was stimulated by infection in all groups. Severe tissue damage was not prevented by EOW treatments. Moderate efficacy may be due to immunomodulatory properties and not to a direct toxic effect on the parasite.
恰加斯病是拉丁美洲一个主要的公共卫生问题。针对……需要混合的Th1/Th2免疫反应。电解氧化水（EOW）已被证明具有杀菌功效。本研究的目的是从临床、免疫学和组织学方面评估EOW对感染的BALB/c小鼠的有效性。通过寄生虫血症、总体健康状况、死亡率、巨型综合征和组织学损伤来评估感染的严重程度。对IgG、肿瘤坏死因子-α、干扰素-γ和白细胞介素-1β水平进行定量。给予EOW对寄生虫血症、总体身体状况和死亡率显示出有益效果。在感染后50天观察到高水平的IgG1。预防性EOW治疗能够在急性期后期基于IgG2a水平的增加诱导主要的TH1免疫反应，并在整个急性期使干扰素-γ增加10倍。EOW控制急性期感染的效果与苯硝唑一样有效。EOW治疗导致脾肿大，并且在所有组中感染刺激了淋巴结病。EOW治疗未能预防严重的组织损伤。中等疗效可能归因于免疫调节特性，而非对寄生虫的直接毒性作用。
