Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo, Japan.
Methods Mol Biol. 2020;2132:597-607. doi: 10.1007/978-1-0716-0430-4_51.
Histo-blood group antigen (HBGA) recognition by norovirus (NoV) has been studied using various techniques. Enzyme-linked immunosorbent assays (ELISAs) using virus-like particles (VLPs) have enabled us to visualize the last step of HBGAs-NoV binding with a total reaction time of approximately 8 h. Herein, we describe two ELISA-based methods to detect and quantify NoV VLP attachment to HBGAs: saliva-VLP binding assay and carbohydrate-VLP binding assay.
组织血型抗原（HBGA）被诺如病毒（NoV）识别的研究已采用多种技术进行。酶联免疫吸附试验（ELISA）使用病毒样颗粒（VLPs）可以使我们直观地观察到 HBGAs-NoV 结合的最后一步，总反应时间约为 8 小时。本文介绍了两种基于 ELISA 的方法来检测和定量 NoV VLP 与 HBGA 的结合：唾液-VLP 结合试验和碳水化合物-VLP 结合试验。
