Ye D X, Zhou X, Yang Q, Hu X Y, Li H, Xiang F G
Department of Pathology, School of Basic Medicine, Qingdao University, Qingdao, 266021, China.
Department of Pathology, the Affiliated Qingdao University Hospital, Qingdao 266003, China.
Zhonghua Gan Zang Bing Za Zhi. 2020 Mar 20;28(3):259-265. doi: 10.3760/cma.j.cn50113-20191104-00405.
To detect the expression of Myc-induced nuclear antigen 53 (Mina53) and liver tissue >5 cm from the edge of the tumor (LTM5), and analyze its relationship with tumorigenesis, clinicopathological characteristics, and patient survival and prognosis in hepatocellular carcinoma (HCC). The expression levels of Mina53 mRNA and protein in 18 pairs of fresh HCC and LTM5 were assessed by qRT-PCR and Western blot, respectively. The expression of Mina53 in 284 pairs of HCC and LTM5 sample was determined by immunohistochemistry. Paired-sample t-test was used for the comparison of measurement data among groups, and heterogeneity of variance was tested using Wilcoxon rank-sum test. (2) test was used for the comparison of measurement data among groups. Kaplan-Meier method and log-rank test were used for survival analysis. Cox regression model was used for single factor and multi factor analysis. The relative expression levels of Mina53 mRNA and protein in 18 fresh HCC tissues were significantly higher than those in LTM5 tissues (mRNA: -4.41 ± 1.48 and -5.93 ± 1.65, = 3.100, = 0.007; protein: 1.12 ± 0.29 and 0.46 ± 0.21, = 10.616, < 0.001). The relative expression level of Mina53 in 284 HCC tissues was higher than that of LTM5 ( = -18.739, < 0.001). The expression level of Mina53 was associated with tumor size ( (2) = 5.474, = 0.019), vascular invasion ( (2) = 8.965, = 0.003), pathological grade ( (2) = 12.006, = 0.002), and TNM stage ( (2) = 16.686, < 0.001). The overall postoperative survival time and disease-free survival time of patients with high expression of Mina53 (28.5 months and 22.7 months, respectively) were shorter than those with low expression (33.0 months and 31.8 months, respectively) ( < 0.05) in HCC. Cox multivariate regression analysis showed that Mina53 and multiple tumors were independent prognostic factors affecting the overall postoperative survival time and disease-free survival time of HCC patients ( < 0.05). Mina53 may play an important role in the occurrence of HCC and participate in the process of tumor growth as well as invasion and metastasis. The high expression of Mina53 signifies that the patient has a poor prognosis and thus can be used as a potential marker for judging the prognosis of HCC patients.
检测Myc诱导的核抗原53（Mina53）及距肿瘤边缘>5 cm肝组织（LTM5）的表达，分析其与肝细胞癌（HCC）发生、临床病理特征及患者生存预后的关系。分别采用qRT-PCR和蛋白质免疫印迹法检测18对新鲜HCC及LTM5中Mina53 mRNA和蛋白的表达水平。采用免疫组织化学法检测284对HCC及LTM5样本中Mina53的表达。组间计量资料比较采用配对样本t检验，方差齐性检验采用Wilcoxon秩和检验。组间计量资料比较采用（2）检验。生存分析采用Kaplan-Meier法和log-rank检验。Cox回归模型用于单因素和多因素分析。18例新鲜HCC组织中Mina53 mRNA和蛋白的相对表达水平显著高于LTM5组织（mRNA：-4.41±1.48和-5.93±1.65，=3.100，=0.007；蛋白：1.12±0.29和0.46±0.21，=10.616，<0.001）。284例HCC组织中Mina53的相对表达水平高于LTM5（=-18.739，<0.001）。Mina53表达水平与肿瘤大小（（2）=5.474，=0.019）、血管侵犯（（2）=8.965，=0.003）、病理分级（（2）=12.006，=0.002）及TNM分期（（2）=16.686，<0.001）相关。HCC中Mina53高表达患者的术后总生存时间和无病生存时间（分别为28.5个月和22.7个月）短于低表达患者（分别为33.0个月和31.8个月）（<0.05）。Cox多因素回归分析显示，Mina53和多发肿瘤是影响HCC患者术后总生存时间和无病生存时间的独立预后因素（<0.05）。Mina53可能在HCC发生中起重要作用，并参与肿瘤生长及侵袭转移过程。Mina53高表达提示患者预后不良，可作为判断HCC患者预后的潜在标志物。
