Wu S S, Yu J N, Jiao J, Chen G S, Zhang C Y, Yu S F
SINOPEC Research Institute of Safty Engineering, Qingdao 266000, China.
Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Feb 20;38(2):128-132. doi: 10.3760/cma.j.issn.1001-9391.2020.02.011.
To investigate the relationship between SNP and noise-induced hearing loss (NIHL) susceptibility in occupational noise exposure population in China. From 6297 for a certain steel works in contact with noise, contact length of 3 years or more and workplace noise exposure intensity of 80 dB (A) , ears or high frequency (3 000, 4 000, 6 000 Hz) average of hearing acuity 40 dB (HL) , or high frequency loss in both ears, on the basis of single whisper frequency (500, 1, 000, 2 000 Hz) average threshold of 26 dB (HL) or object as case group. A case-control study was designed with 1:1 matching. Subjects with the same gender, the same type of work, age ±5 years old, and working age ±2 years after noise exposure were selected as the control group. Subjects with any whisper frequency (500, 1, 000, 2, 000 Hz) whose hearing threshold in any frequency band was ≤25 dB (A) and whose average high-frequency hearing threshold in pure tone hearing test was <35 dB (A) were selected as the control group. Four sites of PON2 gene were genotyped by medium-and high-throughput SNP genotyping. Univariate logistic regression was used to analyze the relationship between single SNP polymorphism and NIHL. A total of 286 case-control pairs were included. Smoking was statistically significant difference between cases and controls (<0.001) . No statistical difference has been found between single SNP polymorphism and NIHL. At the level of greater than 92 dB of high noise exposure, rs7785846 (CT+TT) genotype is a risk factor for occupational noise deafness, and its is 2.74 (95%: 1.09-6.89) compared with wild homozygous type (CC) . Conclusion. The rs7785846 (CT+TT) genotype carriers of PON2 gene are more susceptible to hearing impairment when exposed to high noise intensity.
为研究中国职业噪声暴露人群中SNP与噪声性听力损失（NIHL）易感性之间的关系。从某钢铁厂6297名接触噪声、接触时长3年及以上且工作场所噪声暴露强度为80 dB（A）、双耳或高频（3000、4000、6000 Hz）平均听阈40 dB（HL），或双耳高频听力损失，且单耳低语频率（500、1000、2000 Hz）平均听阈26 dB（HL）及以上者中选取对象作为病例组。采用1:1匹配设计病例对照研究。选取性别相同、工作类型相同、年龄±5岁、噪声暴露后工龄±2年的对象作为对照组。选取任一低语频率（500、1000、2000 Hz）各频段听阈≤25 dB（A）且纯音听力测试中高频平均听阈<35 dB（A）者作为对照组。通过中高通量SNP基因分型对PON2基因的4个位点进行基因分型。采用单因素logistic回归分析单个SNP多态性与NIHL的关系。共纳入286对病例对照。病例组与对照组吸烟情况差异有统计学意义（<0.001）。单个SNP多态性与NIHL之间未发现统计学差异。在高噪声暴露大于92 dB水平时，rs7785846（CT+TT）基因型是职业性噪声聋的危险因素，与野生纯合型（CC）相比，其比值比为2.74（95%：1.09 - 6.89）。结论：PON2基因rs7785846（CT+TT）基因型携带者在高噪声强度暴露时更易发生听力损害。
