Department of Biomedical Science, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran.
Department of Clinical Biochemistry, Abadan Faculty of Medical Sciences, Abadan, Iran.
Iran Biomed J. 2020 Jul;24(4):251-6. doi: 10.29252/ibj.24.4.251. Epub 2020 Feb 10.
The present study aimed to evaluate the effects of different concentrations of cerium oxide nanoparticles (CONPs) on the oxidative stress (OS) status in kidney, lung, and serum of rats.
Male Wistar Rats were treated intraperitoneally with 15, 30, and 60 mg/kg/day of CONPs. The biochemical parameters, including total antioxidant capacity (TAC), total thiol group (TTG), malondialdehyde (MDA), SOD (superoxide dismutase), and catalase (CAT) were assayed in serum, kidney, and lung tissues.
MDA decreased, but TTG and CAT increased in serum by the administration of CONPs at 15 mg/kg. In kidney homogenate obtained from the group treated with CONPs at 15 mg/kg, TAC, TTG, and CAT significantly increased compared to the control group. However, CONPs at 15, 30, and 60 mg/kg significantly decreased MDA level compared to the control group. In lung tissue, CONPs in doses of 15, 30 and 60 mg/kg significantly decreased CAT activity, TTG and TAC compared to the control group, while in kidney tissue, CONPs at the concentrations of 30 and 60 mg/kg significantly increased MDA compared to the control group.
Our findings suggest that CONPs attenuate OS in the kidney and affect the serum levels of OS-related markers but induce OS in the lung tissue in a dose-dependent manner.
本研究旨在评估不同浓度的氧化铈纳米粒子（CONPs）对大鼠肾、肺和血清中氧化应激（OS）状态的影响。
雄性 Wistar 大鼠经腹腔注射给予 15、30 和 60mg/kg/d 的 CONPs。测定血清、肾和肺组织中的生化参数，包括总抗氧化能力（TAC）、总巯基基团（TTG）、丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。
CONPs 以 15mg/kg 给药时，血清中 MDA 减少，而 TTG 和 CAT 增加。与对照组相比，CONPs 以 15mg/kg 处理的肾匀浆中 TAC、TTG 和 CAT 显著增加。然而，与对照组相比，CONPs 以 15、30 和 60mg/kg 显著降低 MDA 水平。在肺组织中，CONPs 在 15、30 和 60mg/kg 的剂量下显著降低 CAT 活性、TTG 和 TAC 与对照组相比，而在肾组织中，CONPs 在 30 和 60mg/kg 的浓度下显著增加 MDA 与对照组相比。
我们的研究结果表明，CONPs 可减轻肾脏的 OS，并影响与 OS 相关标志物的血清水平，但以剂量依赖的方式诱导肺组织中的 OS。
