Behroozi Zahra, Rahimi Behnaz, Hamblin Michael R, Nasirinezhad Farinaz, Janzadeh Atousa, Ramezani Fatemeh
From the Physiology Research Center, Institute of Neuropharmaclogy, Kerman University of Medical Sciences. Kerman, Iran.
Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
J Neuropathol Exp Neurol. 2022 Jul 19;81(8):635-642. doi: 10.1093/jnen/nlac026.
This study investigated the effects of local injection of cerium oxide nanoparticles (CeONPs) in a rat spinal cord injury (SCI) model. Thirty-six adult male Wistar rats were divided into 4 groups: controls (healthy animals), sham (laminectomy), SCI (laminectomy+SCI induction), and treatment (laminectomy+SCI induction+intrathecal injection of CeONPs immediately after injury). SCI was induced using an aneurysm clip at the T12-T13 vertebral region. Motor performance and pain threshold tests were performed weekly; H&E staining and measurement of cavity sizes were performed 6 weeks after injury. The expression of granulocyte colony-stimulating factor (GCSF), P44/42 MAPK, P-P44/42 MAPK, Tau, myelin-associated glycoprotein(MAG) was evaluated after 6 weeks by Western blot. The Basso, Beattie, and Bresnahan locomotor scoring scales improved in animals receiving CeONPs compared with SCI animals. The cavity sizes were less in the treatment group. GCSF expression was similar in the animals receiving CeONPs compared with the SCI group but the expression of ERK1/ERK2 and phospho-ERK was lower than in the SCI group. Expression levels of Tau and MAG were significantly increased in treated animals compared to the SCI group. These data indicate that the use of CeONPs may improve motor functional recovery in SCI.
本研究调查了在大鼠脊髓损伤（SCI）模型中局部注射氧化铈纳米颗粒（CeONPs）的效果。36只成年雄性Wistar大鼠被分为4组：对照组（健康动物）、假手术组（椎板切除术）、SCI组（椎板切除术+SCI诱导）和治疗组（椎板切除术+SCI诱导+损伤后立即鞘内注射CeONPs）。在T12 - T13椎体区域使用动脉瘤夹诱导SCI。每周进行运动性能和疼痛阈值测试；损伤6周后进行苏木精-伊红（H&E）染色和空洞大小测量。6周后通过蛋白质印迹法评估粒细胞集落刺激因子（GCSF）、P44/42丝裂原活化蛋白激酶（MAPK）、磷酸化P44/42 MAPK、微管相关蛋白Tau、髓鞘相关糖蛋白（MAG）的表达。与SCI动物相比，接受CeONPs的动物的Basso、Beattie和Bresnahan运动评分量表有所改善。治疗组的空洞尺寸较小。与SCI组相比，接受CeONPs的动物中GCSF表达相似，但ERK1/ERK2和磷酸化ERK的表达低于SCI组。与SCI组相比，治疗动物中Tau和MAG的表达水平显著增加。这些数据表明，使用CeONPs可能会改善SCI中的运动功能恢复。
