Peng Xing-Rong, Wang Qian, Su Hai-Guo, Zhou Lin, Xiong Wen-Yong, Qiu Ming-Hua
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
University of the Chinese Academy of Sciences, Beijing 100049, China.
J Fungi (Basel). 2022 Mar 22;8(4):331. doi: 10.3390/jof8040331.
Our previous research has shown that lanostane triterpenoids from exhibit significant anti-adipogenesis effects. In order to obtain more structurally diverse lanostane triterpenoids to establish a structure-activity relationship, we continued the study of lanostane triterpenoids from the fruiting bodies of , and forty highly oxygenated lanostane-type triterpenoinds (-), including sixteen new compounds (-), were isolated. Their structures were elucidated using NMR spectra, X-ray crystallographic analysis, and Mosher's method. In addition, some of their parts were evaluated to determine their anti-adipogenesis activities in the 3T3-L1 cell model. The results showed that compounds , , , and exhibited stronger anti-adipogenesis effects than the positive control (LiCl, 20 mM) at the concentration of 20 μM. Compounds and could significantly reduce the lipid accumulation during the differentiation process of 3T3-L1 cells, comparable to the untreated group. Their IC values were 6.42 and 5.39 μM, respectively. The combined results of our previous and present studies allow us to establish a structure-activity relationship of lanostane triterpenoids, indicating that the A--23→26 lactone skeleton could play a key role in anti-adipogenesis activity.
我们之前的研究表明，来自[具体来源未给出]的羊毛甾烷三萜类化合物具有显著的抗脂肪生成作用。为了获得更多结构多样的羊毛甾烷三萜类化合物以建立构效关系，我们继续对[具体来源未给出]子实体中的羊毛甾烷三萜类化合物进行研究，分离得到了40个高度氧化的羊毛甾烷型三萜类化合物（-），其中包括16个新化合物（-）。通过核磁共振光谱、X射线晶体学分析和莫舍尔方法对它们的结构进行了阐明。此外，对它们的部分化合物在3T3-L1细胞模型中评估其抗脂肪生成活性。结果表明，化合物[具体化合物编号未给出]在20 μM浓度下比阳性对照（LiCl，20 mM）表现出更强的抗脂肪生成作用。化合物[具体化合物编号未给出]和[具体化合物编号未给出]在3T3-L1细胞分化过程中能显著降低脂质积累，与未处理组相当。它们的IC值分别为6.42和5.39 μM。我们之前和目前研究的综合结果使我们能够建立羊毛甾烷三萜类化合物的构效关系，表明A--23→²⁶内酯骨架在抗脂肪生成活性中可能起关键作用。
