National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
School of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Harmful Algae. 2020 Mar;93:101791. doi: 10.1016/j.hal.2020.101791. Epub 2020 Apr 1.
Microcystis aeruginosa bloom releases microcystins (MCs) into global aquatic environment, which other living organisms can ingest the released MCs. The toxic effects of MCs on organisms are amplified through the food chain, threatening human and animal health. Lanthanum(III) [La(III)], a pollutant in aquatic environments worldwide, has been confirmed to stimulate MC synthesis in M. aeruginosa. However, the involved cellular mechanism remains unclear. Here, using interdisciplinary approaches, it was first observed that La(III) activated the clathrin-mediated endocytosis in M. aeruginosa. This allowed the algal cells to rapidly absorb macro-elements (C, N and P) and micro-elements (K, Ca and Mg) through the clathrin-mediated endocytosis. These in turn stimulated chlorophyll production, photosynthesis, the growth of the algal cells, and the increases in the productions of MC-LW, MC-LR and MC-YR in M. aeruginosa. These results provide valuable insights for understanding the involved cellular mechanism on MC synthesis and managing MC pollution, which is important to protect global food chain and the ecosystem.
铜绿微囊藻水华将微囊藻毒素（MCs）释放到全球水生环境中，其他生物体会摄入释放的 MCs。MCs 对生物体的毒性作用通过食物链放大，威胁人类和动物健康。镧（III）[La（III）]，全球水生环境中的污染物，已被证实能刺激铜绿微囊藻中 MC 的合成。然而，所涉及的细胞机制仍不清楚。在这里，采用多学科方法，首次观察到 La（III）激活了铜绿微囊藻中的网格蛋白介导的内吞作用。这使得藻类细胞能够通过网格蛋白介导的内吞作用快速吸收宏量元素（C、N 和 P）和微量元素（K、Ca 和 Mg）。这反过来又刺激了叶绿素的产生、光合作用、藻类细胞的生长，以及铜绿微囊藻中 MC-LW、MC-LR 和 MC-YR 的产量增加。这些结果为理解 MC 合成和管理 MC 污染所涉及的细胞机制提供了有价值的见解，这对于保护全球食物链和生态系统非常重要。
