Department of Animal Science, Penn State University, University Park 16802.
Department of Biological Systems Engineering, University of Wisconsin, Madison 53706.
J Dairy Sci. 2020 Jun;103(6):5162-5169. doi: 10.3168/jds.2019-17479. Epub 2020 Apr 16.
Milk yield is a fundamental observation in most dairy experiments and is commonly determined using integrated milk meters that measure milk weight as the cow is being milked. These meters are heavily used in a harsh environment and often are not regularly calibrated, so calibration errors and mechanical problems may create artificial variation in milk weight data. Additionally, direct calibration by collection of milk in a bucket is difficult and imperfect because the use of the bucket may affect yield recorded by the milk meter. The objective of this work was to define a method to easily check parlor meter precision and adjust milk weight values for variation between individual stalls in a parlor. Because most cows are milked in a different stall at each milking, it has been proposed that stall deviations that represent the fixed effect of stall on milk weight could be statistically determined. Individual milk weights from 14 milkings across 7 d from approximately 200 cows were collected from the Penn State dairy farm, which is equipped with a double-10 herringbone parlor with an Afimilk 2000 milking system (S.A.E. Afikim, Afikim, Israel). Milk yield was measured automatically by in-line flow through milk meters (Afi 200; S.A.E. Afikim). The effect of stall on milk weight was modeled using a mixed model that included the fixed effect of stall and the random effects of day, milking time, and cow. First, stall deviations were calculated as the stall least squares means (LSM) minus the average LSM to identify malfunctioning meters requiring service (e.g., deviation exceeding 1 kg). A correction factor for each stall was then generated by dividing the LSM of each stall by the average LSM. Milk yields were then corrected by multiplying the meter weight value by the correction factor. To determine the effect of the correction, raw and corrected meter values were compared with weight of milk collected in a bucket (n = 3/stall). The corrected values had a 5% greater coefficient of determination than raw meter values (0.89 vs. 0.84) and had a lower average percent difference from the bucket milk weight compared with raw meter values (12.6% vs. 13.5%). The method was then used in 3 experiments with 121, 140, and 683 milk yield observations. In all data sets, correcting milk weights slightly improved model fit and had minimal effect on model term standard errors. However, this validation was completed in a parlor where the method was routinely used to identify stalls requiring service; the effect of stall corrections is expected to be larger in parlors without frequent monitoring. Stall deviations are expected to be due predominantly to calibration of the meter but also could be due to differences in pulsation or other stall-specific factors that result in a change in milk yield. It is important to account for these other sources of milk weight variation that are unrelated to treatment. Modeling the effect of stall is a simple, convenient, and low-cost method to monitor and improve milk meter precision and functionality and can be used to reduce artificial variation and experimental error.
产奶量是大多数奶牛实验中的基本观察指标,通常使用集成式牛奶计量器来测量,该计量器在奶牛挤奶时测量牛奶重量。这些计量器在恶劣的环境中大量使用,而且通常不定期校准,因此校准误差和机械问题可能会导致牛奶重量数据出现人为变化。此外,由于使用桶直接收集牛奶会影响计量器记录的产量,因此难以实现且并不完善。本研究的目的是定义一种方法,以方便检查牛舍计量器的精度,并调整牛舍内每个牛位之间牛奶重量的变化。由于大多数奶牛在每次挤奶时都在不同的牛位,因此有人提出,可以通过统计学方法确定代表牛位对牛奶重量的固定影响的牛位偏差。本研究从宾夕法尼亚州立大学奶牛场收集了大约 200 头奶牛在 7 天内的 14 次挤奶的数据,该奶牛场配备了带有 Afimilk 2000 挤奶系统的双 10 型十字形牛舍(S.A.E. Afikim, Afikim,以色列)。牛奶产量通过在线流量式牛奶计量器(Afi 200; S.A.E. Afikim)自动测量。使用包含牛位固定效应和天数、挤奶时间和奶牛随机效应的混合模型来模拟牛位对牛奶重量的影响。首先,通过牛位最小二乘均值(LSM)减去平均 LSM 来计算牛位偏差,以确定需要维修的故障计量器(例如,偏差超过 1kg)。然后,通过将每个牛位的 LSM 除以平均 LSM,为每个牛位生成一个校正因子。然后,通过将计量器的 LSM 值乘以校正因子来校正牛奶产量。为了确定校正的效果,将原始和校正后的计量器值与在桶中收集的牛奶重量(n=3/牛位)进行比较。与原始计量器值相比,校正值的决定系数增加了 5%(0.89 比 0.84),并且与原始计量器值相比,平均百分比差异更小(12.6%比 13.5%)。然后,该方法在 3 项包含 121、140 和 683 个牛奶产量观察值的实验中得到了应用。在所有数据集,校正牛奶重量略微改善了模型拟合度,并且对模型项标准误差的影响最小。然而,这项验证是在一个定期使用该方法来识别需要维修的牛位的牛舍中完成的;在没有频繁监测的牛舍中,牛位校正的效果预计会更大。牛位偏差预计主要归因于计量器的校准,但也可能归因于脉动或其他牛位特定因素的差异,从而导致牛奶产量的变化。很有必要考虑与处理无关的其他牛奶重量变化来源。模拟牛位的影响是一种简单、方便且低成本的监测和提高牛奶计量器精度和功能的方法,可以用来减少人为变化和实验误差。