Li Ying, Wang Ruyuan, Fan Aiping
School of Pharmaceutical Science and Technology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, Tianjin, China.
Anal Sci. 2020 Sep 10;36(9):1075-1079. doi: 10.2116/analsci.20P098. Epub 2020 Apr 17.
A sensitive sensing method was developed for the determination of alkaline phosphatase (ALP) activity based on gold nanocluster (Au NC)-catalyzed luminol-HO chemiluminescent (CL) reaction. The CL signal of luminol-HO-Au NCs can be quenched by ascorbic acid, which was the product of magnesium ascorbyl phosphate (MAP) hydrolysis reaction catalyzed by ALP. The proposed sensing platform showed convenient, sensitive and selective detection of ALP in the range of 0.0027 - 1.3890 U L, with the detection limit of 0.0026 U L. The broad detection linear range and ultra-high sensitivity were inherited from the efficient free radical scavenging capability of ascorbic acid on the luminol-HO-Au NCs CL reaction. The CL sensing platform was applied to the detection of ALP activity in serum samples. We believe that this sensing platform is a universal CL strategy for ALP detection because ascorbic acid is an efficient CL quencher for many CL reactions.
基于金纳米簇（Au NC）催化的鲁米诺-H₂O₂化学发光（CL）反应，开发了一种用于测定碱性磷酸酶（ALP）活性的灵敏传感方法。鲁米诺-H₂O₂-Au NCs的CL信号可被抗坏血酸淬灭，抗坏血酸是碱性磷酸酶催化的抗坏血酸磷酸镁（MAP）水解反应的产物。所提出的传感平台在0.0027 - 1.3890 U/L范围内对ALP具有方便、灵敏和选择性的检测能力，检测限为0.0026 U/L。宽检测线性范围和超高灵敏度源于抗坏血酸对鲁米诺-H₂O₂-Au NCs CL反应的高效自由基清除能力。该CL传感平台应用于血清样品中ALP活性的检测。我们认为，这种传感平台是一种通用的ALP检测CL策略，因为抗坏血酸是许多CL反应的有效CL淬灭剂。
