Department of Applied Chemistry, Cochin University of Science and Technology, Kochi, 682022, Kerala, India.
Anal Sci. 2021 Apr 10;37(4):599-603. doi: 10.2116/analsci.20P322. Epub 2020 Oct 16.
For the first time, we report on a copper nanoclusters based fluorescence sensor for hemoglobin (Hgb). The aggregation-induced quenching of tannic acid capped copper nanoclusters' (TACuNCs) fluorescence by a Hgb-HO mixture that mimics the Fenton's reagent is used here for the selective determination of Hgb. It is possible to effectively determine Hgb using this sensitive and cost-effective sensor in the linear range of 5.0 × 10 to 4.0 × 10 M with a detection limit of 5.6 × 10 M. The practical utility of the sensor is evident from the good recovery values obtained from Hgb spiked with artificial blood serum.
我们首次报道了一种基于铜纳米簇的荧光传感器，用于检测血红蛋白（Hgb）。在此，我们利用模拟 Fenton 试剂的 Hgb-HO 混合物使单宁酸包覆的铜纳米簇（TACuNCs）的荧光发生聚集诱导猝灭，用于选择性测定 Hgb。该传感器具有较高的灵敏度和成本效益，在 5.0×10^-5~4.0×10^-4 M 的线性范围内可有效测定 Hgb，检测限为 5.6×10^-5 M。通过在人工血清中加入 Hgb 进行实际应用验证，该传感器具有良好的回收率。
