Mokhtarzadeh Elham, Abolhasani Jafar, Hassanzadeh Javad
Department of Chemistry, Tabriz Branch, Islamic Azad University.
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz.
Anal Sci. 2019 May 10;35(5):543-550. doi: 10.2116/analsci.18P532. Epub 2019 Jan 18.
The less stability and robustness, high-cost preparation and maintenance of natural enzymes, especially horseradish peroxidase (HRP), challenge researchers to introduce effective alternatives for their wide applications. Herein, the peroxidase-like activity of AuCu bimetal nanoclusters (AuCu NCs) was investigated in the rhodamine B-HO chemiluminescence (CL) system. AuCu NCs could effectively catalyzed the CL reaction, and a high intensive emission intensity was obtained. A comprehensive study was implemented to examine the effects of different stabilizing ligands and Au/Cu ratios on the catalytic activity of obtained NCs. Comparison experiments were also expanded to include Au and Cu nanoparticles with different sizes, too. The results verified the superior catalytic activity of penicillamine-stabilized AuCu bimetal NCs containing 50% cu atoms. Finally, the analytical application of the introduced CL system showed great sensitivity for HO detection, with a detection limit of 0.13 nM. Moreover, the developed CL method was able to measure glucose and xanthine over wide concentration ranges of 0.1 - 400 and 0.1 - 200 μM, respectively. The method also indicated satisfactory reliability, confirmed by standard reference materials.
天然酶，尤其是辣根过氧化物酶（HRP），稳定性和鲁棒性较差，制备和维护成本高，这给研究人员带来挑战，促使他们寻找有效的替代物以实现其广泛应用。在此，研究了金铜双金属纳米簇（AuCu NCs）在罗丹明B-过氧化氢（HO）化学发光（CL）体系中的类过氧化物酶活性。AuCu NCs能够有效催化CL反应，并获得高强度发射强度。开展了一项综合研究，考察不同稳定配体和金/铜比例对所得纳米簇催化活性的影响。对比实验还扩展到包括不同尺寸的金和铜纳米颗粒。结果证实了含50%铜原子的青霉胺稳定化金铜双金属纳米簇具有优异的催化活性。最后，所引入的CL体系的分析应用对HO检测表现出极高的灵敏度，检测限为0.13 nM。此外，所开发的CL方法能够分别在0.1 - 400 μM和0.1 - 200 μM的宽浓度范围内测定葡萄糖和黄嘌呤。该方法还显示出令人满意的可靠性，经标准参考物质验证。
