Department of Biology, Faculty of Arts and Sciences, Sakarya University, 54050, Serdivan, Sakarya, Turkey.
Department of Biology, Faculty of Science, Ege University, 35100, Bornova, Izmir, Turkey.
Environ Sci Pollut Res Int. 2020 Jun;27(18):22423-22433. doi: 10.1007/s11356-020-08853-7. Epub 2020 Apr 20.
Acrolein is a widely distributed pollutant produced from various sources such as industrial waste, organic combustion, and power plant emissions. It is also intentionally released into irrigation canals to control invasive aquatic plants. Zebrafish (Danio rerio) has a good reputation for being an attractive model organism for developmental and toxicological research. In this study, zebrafish embryos were exposed to acrolein to investigate the cardiotoxic effects. The 96-h LC (median lethal concentration) value of acrolein was determined as 654.385 μg/L. Then, the embryos were treated with the sublethal experimental concentrations of acrolein (1, 4, 16, 64, and 256 μg/L) for 96 h. Embryos were examined at 48, 72, and 96 h post-fertilization (hpf). Acrolein affected the cardiac morphology and function of the embryos. Sinus venosus-bulbus arteriosus (SV-BA) distance of 64 μg/L and 256 μg/L acrolein groups was elongated compared with the control samples. Immunostaining with MF20 antibody clearly exhibited that the atrium positioned posterior to the ventricle which indicated cardiac looping inhibition. Histological preparations also showed the mispositioning and the lumens of the chambers narrowed. Acrolein-induced increased heart rate was noted in the 4, 16, 64, and 256 μg/L treatment groups. Taken together, these results indicated that acrolein disrupted the heart development and cardiac function in zebrafish, suggesting that its water-borne risks should be considered seriously.
丙烯醛是一种广泛分布的污染物，由工业废物、有机燃烧和电厂排放等各种来源产生。它也被故意释放到灌溉渠道中以控制入侵水生植物。斑马鱼（Danio rerio）作为发育和毒理学研究的有吸引力的模式生物而享有盛誉。在这项研究中，斑马鱼胚胎暴露于丙烯醛中，以研究其心脏毒性作用。丙烯醛的 96-h LC（半数致死浓度）值确定为 654.385μg/L。然后，将胚胎用亚致死实验浓度的丙烯醛（1、4、16、64 和 256μg/L）处理 96h。胚胎在受精后 48、72 和 96h（hpf）进行检查。丙烯醛影响胚胎的心脏形态和功能。64μg/L 和 256μg/L 丙烯醛组的窦房结-动脉球（SV-BA）距离比对照样品延长。用 MF20 抗体进行免疫染色清楚地显示出心房位于心室后方，表明心脏环化抑制。组织学制备也显示出错位和腔室的管腔变窄。在 4、16、64 和 256μg/L 处理组中观察到丙烯醛诱导的心率增加。综上所述，这些结果表明丙烯醛破坏了斑马鱼的心脏发育和心脏功能，表明应认真考虑其水传播风险。
