Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, China.
Ocean College, Hainan University, Haikou, P. R. China.
Vet Med Sci. 2020 Aug;6(3):649-657. doi: 10.1002/vms3.267. Epub 2020 Apr 19.
Gut microbiota in fish plays an important role in the nutrient digestion, immune responses and disease resistance. To understand the effect of fluoroquinolone antibiotic bath administration on fish gut microbiota, the gut microbiota community in the coral trout Plectropomus leopardus (Lacepède, 1802) was studied after enrofloxacin bathing treatment at two concentrations (5 and 10 mg/L) and 0 mg/L as control. A total of 90 fish were used in this study, and three replicates were used for each treatment. After a 24-hr bath, the gut bacterial composition was analyzed using high-throughput Illumina sequencing. The results indicated that the richness, diversity and the dominant bacterial taxa of P. leopardus gut bacteria were not affected by enrofloxacin bathing (p > .05). Proteobacteria and Firmicutes were the dominant phyla, and Exiguobacterium, Citrobacter, Vibrio, Acinetobacter, Pseudomonas were the dominant genus. The findings in the present study provide an understanding on the relationship between fish gut bacteria community and antibiotic bath administration. The findings of this study are instructive on the antibiotic bath administration applied for the management of P. leopardus health in aquaculture.
鱼类肠道微生物在营养消化、免疫反应和疾病抵抗力方面发挥着重要作用。为了了解氟喹诺酮类抗生素浸浴处理对鱼类肠道微生物群的影响，本研究在两个浓度（5 和 10mg/L）和 0mg/L 作为对照的情况下，研究了珊瑚斑 Plectropomus leopardus（ Lacepède，1802）肠道微生物群的组成。本研究共使用了 90 条鱼，每个处理组使用 3 个重复。浸浴 24 小时后，使用高通量 Illumina 测序分析肠道细菌组成。结果表明，恩诺沙星浸浴处理并未影响 P. leopardus 肠道细菌的丰富度、多样性和主要细菌类群（p>.05）。变形菌门和厚壁菌门是主要的门，优势属为节杆菌属、柠檬酸杆菌属、弧菌属、不动杆菌属和假单胞菌属。本研究的结果提供了对鱼类肠道细菌群落与抗生素浸浴处理之间关系的理解。本研究结果对水产养殖中用于管理 P. leopardus 健康的抗生素浸浴处理具有指导意义。
