Department of Stomatology, Jingmen No.1 People's Hospital, Jingmen, China.
J Recept Signal Transduct Res. 2020 Oct;40(5):442-448. doi: 10.1080/10799893.2020.1752718. Epub 2020 Apr 20.
Alveolar bone has a high plasticity. The health of alveolar bones directly determines the success of oral implant surgery, and the health and functions of oral and maxillofacial system. Hesperidin (HES) has many pharmacological activities, such as anti-inflammatory, anti-oxidation, promotion of osteoblast differentiation, but its effect on alveolar osteoblasts is rarely reported. Healthy human alveolar osteoblasts were treated by HES (0.1, 1, 10, 100 μmol/l) and Wnt signaling pathway inhibitor Dickkopf-1 (DKK-1). Then the cell osteogenic differentiation was detected by Alizarin Red S staining, and alkaline phosphatase (ALP) activity was detected by ALP kit. Expressions of genetic markers such as runt-related transcription factor 2 (RUNX2), bone morphogenetic protein-2 (BMP2), osterix (OSX), and osteocalcin (OCN) of osteoblasts were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Expressions of Wnt/β-Catenin pathway-related proteins were measured by Western blot (WB) analysis. HES increased numbers of the cells stained red by Alizarin Red S staining, the ALP activity, and the mRNA expression of Runx2, BMP2, OSX, and OCN, and activated the Wnt/β-catenin signaling pathway. However, DKK-1 reversed the differentiation function of human alveolar osteoblasts accelerated by HES. HES promoted the differentiation of human alveolar osteoblasts through the activation of the Wnt/β-Catenin signaling pathway.
牙槽骨具有很高的可塑性。牙槽骨的健康状况直接决定了口腔种植手术的成败，以及口腔颌面系统的健康和功能。橙皮苷（HES）具有多种药理活性，如抗炎、抗氧化、促进成骨细胞分化等，但对牙槽骨成骨细胞的作用鲜有报道。用 HES（0.1、1、10、100μmol/L）和 Wnt 信号通路抑制剂 Dickkopf-1（DKK-1）处理健康人牙槽骨成骨细胞，然后用茜素红 S 染色法检测细胞成骨分化，碱性磷酸酶（ALP）试剂盒检测 ALP 活性。通过实时定量聚合酶链反应（qRT-PCR）检测成骨细胞基因标志物 runt 相关转录因子 2（RUNX2）、骨形态发生蛋白 2（BMP2）、osterix（OSX）和骨钙素（OCN）的表达。用 Western blot（WB）分析检测 Wnt/β-catenin 通路相关蛋白的表达。HES 增加了茜素红 S 染色细胞的数量、ALP 活性以及 Runx2、BMP2、OSX 和 OCN 的 mRNA 表达，并激活了 Wnt/β-catenin 信号通路。然而，DKK-1 逆转了 HES 加速的人牙槽骨成骨细胞的分化功能。HES 通过激活 Wnt/β-catenin 信号通路促进人牙槽骨成骨细胞的分化。
