Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Departamento de Fisiologia e Farmacodinâmica, Laboratório de Toxicologia, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil.
Parasitology. 2020 Aug;147(9):932-939. doi: 10.1017/S0031182020000578. Epub 2020 Apr 20.
The co-infection between visceral leishmaniasis (VL) and human immunodeficiency virus (HIV) has increased in several countries in the world. The current serological tests are not suitable since they present low sensitivity to detect the most of VL/HIV cases, and a more precise diagnosis should be performed. In this context, in the present study, an immunoproteomics approach was performed using Leishmania infantum antigenic extracts and VL, HIV and VL/HIV patients sera, besides healthy subjects samples; aiming to identify antigenic markers for these clinical conditions. Results showed that 43 spots were recognized by antibodies in VL and VL/HIV sera, and 26 proteins were identified by mass spectrometry. Between them, β-tubulin was expressed, purified and tested in ELISA experiments as a proof of concept for validation of our immunoproteomics findings and results showed high sensitivity and specificity values to detect VL and VL/HIV patients. In conclusion, the identified proteins in the present work could be considered as candidates for future studies aiming to improvement of the diagnosis of VL and VL/HIV co-infection.
内脏利什曼病（VL）和人类免疫缺陷病毒（HIV）的合并感染在世界上的几个国家有所增加。目前的血清学检测并不适用，因为它们对大多数 VL/HIV 病例的检测灵敏度较低，需要进行更精确的诊断。在这种情况下，本研究采用利什曼原虫抗原提取物和 VL、HIV 和 VL/HIV 患者血清以及健康受试者样本进行免疫蛋白质组学研究，旨在鉴定这些临床情况的抗原标志物。结果表明，VL 和 VL/HIV 血清中的抗体识别出 43 个斑点，通过质谱鉴定出 26 种蛋白质。其中，β-微管蛋白被表达、纯化，并在 ELISA 实验中进行了测试，作为验证我们免疫蛋白质组学研究结果的概念验证，结果显示出对检测 VL 和 VL/HIV 患者的高灵敏度和特异性。总之，本工作中鉴定的蛋白质可被视为未来研究的候选者，旨在提高 VL 和 VL/HIV 合并感染的诊断。
