山竹（Linn.）果皮提取物中氧杂蒽酮和单宁对BHK - 21成纤维细胞培养的比较毒性
The Comparative Toxicity of Xanthones and Tannins in Mangosteen ( Linn.) Pericarp Extract against BHK-21 Fibroblast Cell Culture.
作者信息
Puteri Fikarini H, Widjaja Jennifer, Cahyani Febriastuti, Mooduto Latief, Wahjuningrum Dian Agustin
机构信息
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
出版信息
Contemp Clin Dent. 2019 Apr-Jun;10(2):319-323. doi: 10.4103/ccd.ccd_579_18.
OBJECTIVE
The objective of this study is to compare the toxicity level of xanthones and tannins derived from mangosteen pericarp extract at specific concentrations against BHK-21 fibroblast cell cultures.
METHODS
Mangosteen was extracted using a maceration method with ethanol 96%. Xanthones were isolated from the chloroform extract, whereas tannins were isolated using acetone alcohol and serial diluted to 100% concentration. Toxicity levels were monitored after 24 h using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay technique by ELISA reader at 620 nm.
RESULTS
Viable cells of BHK-21 against xanthone concentration began to decrease (40.24%) at 3.98% xanthones, whereas viable cells of BHK-21 against tannin concentration began to decrease (68.06%) at 2.2% tannins.
CONCLUSION
It is suggested that tannins were more toxic than the xanthones derived from mangosteen pericarp.
目的
本研究的目的是比较山竹果皮提取物中特定浓度的氧杂蒽酮和单宁对BHK - 21成纤维细胞培养物的毒性水平。
方法
采用96%乙醇浸渍法提取山竹。从氯仿提取物中分离出氧杂蒽酮，而单宁则用丙酮醇分离并连续稀释至100%浓度。24小时后，使用酶标仪在620nm波长下通过3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基溴化四氮唑（MTT）检测技术监测毒性水平。
结果
当氧杂蒽酮浓度为3.98%时，BHK - 21的活细胞开始减少（40.24%），而当单宁浓度为2.2%时，BHK - 21的活细胞开始减少（68.06%）。
结论
表明单宁比山竹果皮中的氧杂蒽酮毒性更大。
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