优化麻疯树籽油纳米乳作为一种有潜力的创伤愈合剂。
Optimisation of Calophyllum inophyllum seed oil nanoemulsion as a potential wound healing agent.
机构信息
College of Engineering, IT & Environment, Charles Darwin University, Casuarina campus, Darwin City, 0810, Australia.
Menzies School of Health Research, Charles Darwin University, Darwin City, Australia.
出版信息
BMC Complement Med Ther. 2022 Nov 4;22(1):285. doi: 10.1186/s12906-022-03751-6.
BACKGROUND
Efficient delivery systems of Calophyllum inophyllum seed oil (CSO) in the form of nanoemulsion were optimised to enhance its stability and ensure its therapeutic efficiency as a potential agent for various biomedical applications.
METHOD
Response Surface Methodology (RSM) was used to determine the effects of independent variables (oil, surfactant, water percentage and homogenisation time) on physicochemical characteristics, including droplet size, polydispersity index and turbidity.
RESULTS
The optimised CSO nanoemulsion (CSONE) has a 46.68 nm particle size, 0.15 Polydispersity index value and 1.16 turbidity. After 4 weeks of storage at 5 ± 1 °C and 25 ± 1 °C, the CSONE was physically stable. The optimised CSO nanoemulsion showed enhancement in cell viability and wound healing in baby hamster kidney a clone BHK-21 (BSR) cells as compared to the CSO. The wound healing property of CSONE was higher than CSO.
CONCLUSION
Thus, our in vitro wound healing results demonstrated that CSO in the nanoemulsion form can promote wound healing by enhancing the proliferation and migration of epidermal cells. The coarse emulsion of Calophyllum inophyllum seed oil nano emulsion was prepared using high shear homogeniser techniques. The optimised CSONE with the droplet size of 46.68 nm was prepared from a mixture of CSO, Tween 80, and high pure water (HPW), then used for the biological investigation. The in vitro cell monolayer scratch assay revealed that CSONE in the lowest concentration of CSO resulted in 100% wound closure after 48 hrs. The optimised CSO nanoemulsion was found to be a promising and effective approach in the treatment of wounds by boosting the proliferation and migration of epidermal cells.
背景
为了提高稳定性并确保其作为各种生物医学应用的潜在药物的治疗效果，以纳米乳液的形式对麻疯树籽油（CSO）的有效传递系统进行了优化。
方法
响应面法（RSM）用于确定独立变量（油，表面活性剂，水百分比和匀浆时间）对包括粒径，多分散指数和浊度在内的物理化学特性的影响。
结果
优化的 CSO 纳米乳液（CSONE）的粒径为 46.68nm，多分散指数值为 0.15，浊度为 1.16。在 5±1°C 和 25±1°C 下储存 4 周后，CSONE 在物理上是稳定的。与 CSO 相比，优化的 CSO 纳米乳液在 BHK-21（BSR）细胞中的细胞活力和伤口愈合方面均表现出增强。CSONE 的伤口愈合性能高于 CSO。
结论
因此，我们的体外伤口愈合结果表明，CSO 以纳米乳液形式可以通过增强表皮细胞的增殖和迁移来促进伤口愈合。麻疯树籽油纳米乳液的粗乳液是使用高剪切匀浆技术制备的。从 CSO，吐温 80 和高纯水（HPW）的混合物中制备了粒径为 46.68nm 的最佳 CSONE，然后用于生物学研究。体外单层细胞划痕实验表明，CSONE 在最低 CSO 浓度下，在 48 小时后即可实现 100％的伤口闭合。优化的 CSO 纳米乳液有望成为一种有前途且有效的治疗方法，可通过促进表皮细胞的增殖和迁移来治疗伤口。
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