Shi Rui, Wu Peng, Liu Miaomiao, Chen Bing, Cong Longjiao
Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, People's Republic of China.
Onco Targets Ther. 2020 Apr 8;13:3025-3037. doi: 10.2147/OTT.S237559. eCollection 2020.
Long non-coding RNAs (lncRNAs) have been reported to play essential roles in regulating the radiosensitivity of cancers. Prostate cancer-associated transcript 6 () exerts oncogenic roles in several tumors. However, the roles of and its underlying mechanism in regulating the radiosensitivity of triple-negative breast cancer (TNBC) have not been investigated.
The expression levels of () and tumor protein D52 () were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, apoptosis and colony formation were assessed by Cell Counting Kit-8 (CCK-8) assay, flow cytometry and colony formation assay, respectively. The interaction between and or was predicted by bioinformatics analysis and verified by dual-luciferase reporter assay. Western blot was carried out to detect the protein level of TPD52.
and were highly expressed and was lowly expressed in TNBC tissues and cells, which was associated with an aggressive tumor phenotype in patients, affecting lymph node metastasis and clinical stage. or knockdown or overexpression enhanced the radiosensitivity of TNBC cells via inhibiting proliferation and inducing apoptosis. directly interacted with and negatively regulated expression. Moreover, was confirmed as a target of . Besides, regulated the radiosensitivity of TNBC cells through acting as a molecular sponge of to modulate expression.
Knockdown of promoted the radiosensitivity of TNBC cells through regulating / axis, providing a vital theoretical basis to improve the radiotherapy efficiency of TNBC.
据报道，长链非编码RNA（lncRNAs）在调节癌症放射敏感性方面发挥着重要作用。前列腺癌相关转录本6（ ）在多种肿瘤中发挥致癌作用。然而， 及其在调节三阴性乳腺癌（TNBC）放射敏感性中的潜在机制尚未得到研究。
通过定量实时聚合酶链反应（qRT-PCR）测定 （ ）和肿瘤蛋白D52（ ）的表达水平。分别通过细胞计数试剂盒-8（CCK-8）测定、流式细胞术和集落形成试验评估细胞活力、凋亡和集落形成。通过生物信息学分析预测 与 或 之间的相互作用，并通过双荧光素酶报告基因试验进行验证。进行蛋白质印迹法检测TPD52的蛋白水平。
在TNBC组织和细胞中， 和 高表达， 低表达，这与患者侵袭性肿瘤表型相关，影响淋巴结转移和临床分期。 或 敲低或 过表达通过抑制增殖和诱导凋亡增强了TNBC细胞的放射敏感性。 直接与 相互作用并负向调节 表达。此外， 被确认为 的靶标。此外， 通过作为 的分子海绵调节 表达来调节TNBC细胞的放射敏感性。
敲低 通过调节 / 轴促进TNBC细胞的放射敏感性，为提高TNBC放疗效率提供了重要的理论依据。
