Department of Radiation Therapy, Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Cancer and Basic Medicine (ICBM), Chinese Academy of Sciences, No.1, Banshan East Road, Gongshu District, Hangzhou, 310021, China.
Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (Lung and Esophagus), Hangzhou, 310021, China.
Mol Biotechnol. 2022 Sep;64(9):1003-1012. doi: 10.1007/s12033-022-00475-3. Epub 2022 Mar 23.
Aberrant gene expression caused by miRNAs disorders plays a relevant role in multiple steps of tumorigenesis. In this attempt, we studied the functional role of miR-125b-5p and TPD52 in breast cancer. TPD52 mRNA and miR-125b-5p levels were assessed via qRT-PCR, and TPD52 protein level was analyzed via western blot. By performing CCK-8, transwell invasion and wound healing assays, the phenotype changes in breast cancer cells were assessed. miR-125b-5p was proven as an upstream miRNA of TPD52 in breast cancer via TargetScan database, luciferase activity, and western blot. MiR-125b-5p was prominently decreased while TPD52 was dramatically increased in breast cancer cells. Functional assays exhibited that forced level of TPD52 facilitated cell proliferation, invasion and migration in breast cancer. In the end, the rescue assay proved that miR-125b-5p was a cancer repressor and modulated breast cancer progression by targeting TPD52. All above offer potential biomarkers for breast cancer treatment.
miRNA 紊乱引起的异常基因表达在肿瘤发生的多个步骤中发挥相关作用。在本研究中，我们研究了 miR-125b-5p 和 TPD52 在乳腺癌中的功能作用。通过 qRT-PCR 评估 TPD52 mRNA 和 miR-125b-5p 水平，通过 Western blot 分析 TPD52 蛋白水平。通过 CCK-8、Transwell 侵袭和划痕愈合实验评估乳腺癌细胞表型变化。通过 TargetScan 数据库、荧光素酶活性和 Western blot 证实 miR-125b-5p 是乳腺癌中 TPD52 的上游 miRNA。miR-125b-5p 在乳腺癌细胞中明显降低，而 TPD52 则明显增加。功能实验表明，强制水平的 TPD52 促进了乳腺癌细胞的增殖、侵袭和迁移。最后，挽救实验证明 miR-125b-5p 是一种癌症抑制剂，通过靶向 TPD52 调节乳腺癌的进展。所有这些都为乳腺癌的治疗提供了潜在的生物标志物。
