Popov Todor A, Emberlin Jean, Josling Peter, Seifalian Alexander
University Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia, Bulgaria.
Allergy UK, Sidcup, Kent, UK.
Med Devices (Auckl). 2020 Mar 30;13:107-113. doi: 10.2147/MDER.S236104. eCollection 2020.
Insufflation of powder hydroxypropylmethylcellulose (pHPMC) in the nose has been proven an effective barrier in subjects with rhinitis in many clinical studies. We conducted additionally in vitro and in vivo experiments to address outstanding efficacy and safety issues.
We used an experimental setup to demonstrate the inhibition of the diffusion of allergen extracts (house dust mite, Japanese cedar, Ragweed, Timothy grass) and pollutants (particulate matter 2.5 µm, PM2.5). Safety of pHPMC when insufflated in the airways of rats was assessed in 24 animals which were sacrificed; tissue sections from lungs, brain and liver were made 1, 24 and 48 hrs after pHPMC inhalation and compared to those of control animals.
pHPMC acted as an effective barrier to diffusion of both the liquid allergen extracts and of PM2.5 into the agar covered slides: the quantities of the other tested allergens ranged between <0.5% and 14% of the quantities diffused in the void slides after 6 hrs. The quantity of PM2.5 penetrating the agar was reduced by 94%. Histological photomicrographs did not reveal any evidence of inflammation at 1, 24 and 48 hrs after pHPMC insufflation.
Use of pHPMC should be viewed as a barrier enforcing measure against inhalatory ambient intruders.
在许多临床研究中，已证实向鼻腔内吹入粉末状羟丙基甲基纤维素（pHPMC）对鼻炎患者是一种有效的屏障。我们还进行了体外和体内实验，以解决尚未解决的疗效和安全性问题。
我们采用一种实验装置来证明对变应原提取物（屋尘螨、日本柳杉、豚草、梯牧草）和污染物（2.5微米颗粒物，PM2.5）扩散的抑制作用。对24只处死的大鼠评估了在气道内吹入pHPMC时的安全性；在吸入pHPMC后1、24和48小时制作肺、脑和肝的组织切片，并与对照动物的组织切片进行比较。
pHPMC对液体变应原提取物和PM2.5扩散到琼脂覆盖载玻片起到了有效的屏障作用：6小时后，其他测试变应原扩散的量在空白载玻片扩散量的<0.5%至14%之间。穿透琼脂的PM2.5量减少了94%。组织学显微照片未显示在吹入pHPMC后1、24和48小时有任何炎症迹象。
应将使用pHPMC视为一种针对吸入性环境入侵者的屏障强化措施。
