Ovrebø S, Jacobsen D, Sejersted O M
J Chromatogr. 1987 Apr 24;416(1):111-7. doi: 10.1016/0378-4347(87)80490-5.
Conditions for isotachophoretic determination of anionic metabolites in blood from ethylene glycol in poisoned humans were established. Leading electrolytes with 5 mM chloride and and 2.5 gave good separation. Optimal separation was found with leading electrolytes at pH 2.5, and 10 mM acetic acid as terminating electrolyte. Separation and quantification of four out of six metabolites were possible. The four were glycolic acid, glyoxylic acid, oxalic acid and formic acid. Besides these compounds, citric acid, lactic acid, and alpha- and beta-hydroxybutyric acid were separated and quantified. The formation of mixed zones did not give any serious problems, although in samples with high amounts of glycolic acid we had to reduce the maximum injected amount from 3 to 1 microliter. This method might be valuable in further studies of the mechanism of ethylene glycol toxicity and as an important supplement in the diagnosis of late stages of ethylene glycol poisoning in which the glycol has been metabolized to glycolic acid.
建立了用于中毒人体血液中乙二醇阴离子代谢物等速电泳测定的条件。含5 mM氯化物和2.5的前导电解质实现了良好的分离。发现pH值为2.5的前导电解质和10 mM乙酸作为终止电解质时能实现最佳分离。六种代谢物中的四种能够实现分离和定量。这四种分别是乙醇酸、乙醛酸、草酸和甲酸。除了这些化合物外，柠檬酸、乳酸以及α-和β-羟基丁酸也得到了分离和定量。混合区的形成并未带来严重问题，不过在乙醇酸含量高的样品中，我们不得不将最大进样量从3微升降至1微升。该方法对于进一步研究乙二醇毒性机制可能具有重要价值，并且作为乙二醇已代谢为乙醇酸的乙二醇中毒晚期诊断的重要补充手段。
