Escobedo Humberto D, Stansbury Jeffrey W, Nair Devatha P
Department of Pharmaceutical Science, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, 12850 E. Montview Blvd., Mail Stop C238, Aurora, CO, 80045, USA.
Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus, RC1-South, L18-1101, Mail Stop 8120, 12801 E. 17th Avenue, Aurora, CO, 80045, USA; Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80309, USA.
J Mech Behav Biomed Mater. 2020 Aug;108:103755. doi: 10.1016/j.jmbbm.2020.103755. Epub 2020 Apr 7.
A simple, yet powerful approach to synthesize photoreactive nanogel networks <5 nm that can swell between ~3 and ~200 times their initial radius with control over the size and surface charge via a solution polymerization reaction protocol was demonstrated. Nanogels with hydrodynamic radii from 0.9 nm to 3.2 nm and surface charges from -6.4 mV to -16.5 mV with dramatically different abilities to swell were synthesized by altering the solvent ratio before synthesis. Additionally, the control over the release kinetics of a small molecule over a period of 30 days was demonstrated by the methacrylate functionalization of the nanogels post-synthesis and the subsequent photo-aggregation of the nanogels. Thepotential to control the release of small molecule drugs via the concentration of photoreactive groups and the photo-induced aggregation of the nanogels offers the unique ability to tailor the in situ release kinetics of the delivery network.
展示了一种简单而有效的方法来合成光反应性纳米凝胶网络，该网络尺寸小于5纳米，通过溶液聚合反应方案可在初始半径的约3至约200倍之间膨胀，且能控制其尺寸和表面电荷。通过在合成前改变溶剂比例，合成了流体动力学半径从0.9纳米到3.2纳米、表面电荷从-6.4毫伏到-16.5毫伏且具有显著不同膨胀能力的纳米凝胶。此外，通过合成后纳米凝胶的甲基丙烯酸酯功能化以及随后的纳米凝胶光聚集，展示了对小分子在30天内释放动力学的控制。通过光反应性基团的浓度和纳米凝胶的光诱导聚集来控制小分子药物释放的潜力，为定制递送网络的原位释放动力学提供了独特的能力。
