Ural Federal University Named After the First President of Russia B. N. Yeltsin, Chemical Technological Institute, 19 Ul. Mira, 620002, Ekaterinburg, Russian Federation.
Ural Federal University Named After the First President of Russia B. N. Yeltsin, Chemical Technological Institute, 19 Ul. Mira, 620002, Ekaterinburg, Russian Federation.
Anal Chim Acta. 2020 May 15;1111:83-91. doi: 10.1016/j.aca.2020.03.041. Epub 2020 Mar 27.
An analytical methodology is proposed for a comprehensive study of antioxidant properties of various objects based on the implementation of various mechanisms of antioxidant action. In order to interpret results regarding the action of antioxidants in living organisms it is proposed to adhere to certain requirements for research methods of antioxidant properties, for oxidizing agents of a radical and non-radical nature. The developed integrated approach consists in using the potentiometry method and is based on the antioxidant capacity determination which is founded on the interaction of antioxidants with the К[Fe(CN)] by the ET-mechanism and the conjugation mechanism and the antiradical capacity determination based on the interaction of antioxidants with peroxyl radicals by the HAT-mechanism. Individual antioxidants of natural origin, plant materials extracts as well as synthesized 6-nitro-1,2,4-triazoloazin containing polyphenols fragments, which are complex conjugated structures, have been investigated. It has been shown that in the antioxidants study it is advisable to use the integrated approach that will allow to evaluate antioxidant properties from the point of view of various antioxidants action mechanisms.
提出了一种基于各种抗氧化作用机制实施的方法，用于全面研究各种对象抗氧化性能的分析方法。为了解释抗氧化剂在生物体中的作用的结果，建议坚持研究抗氧化性能的方法的某些要求，以及自由基和非自由基氧化剂。所开发的综合方法包括使用电位法，并基于抗氧化能力的确定，该确定基于抗氧化剂与 K[Fe(CN)]的相互作用，通过 ET 机制和共轭机制，以及基于抗氧化剂与过氧自由基的相互作用的抗自由基能力的确定，通过 HAT 机制。研究了天然来源的个别抗氧化剂、植物材料提取物以及合成的含有多酚片段的 6-硝基-1,2,4-三唑嗪，它们是复杂的共轭结构。结果表明，在抗氧化剂研究中，使用综合方法是明智的，该方法将允许从各种抗氧化剂作用机制的角度评估抗氧化性能。
