Department of Periodontology, Faculty of Dentistry, Gazi University, 8. Cadde 82. Sokak Emek, 06510, Ankara, Turkey.
Department of Medical Microbiology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Odontology. 2021 Jan;109(1):103-113. doi: 10.1007/s10266-020-00520-0. Epub 2020 Apr 20.
The aim of this trial was to analyze the effect of implant surface decontamination procedures combined with reconstructive surgical treatment (RST) of peri-implantitis on gene expression levels of selected biomarkers in peri-implant crevicular fluid (PICF). Forty patients diagnosed with peri-implantitis were treated with RST + decontamination of the implant surface using sterile saline and ozone therapy (ozone group) or sterile saline alone (control group). The gene expression levels of interleukin (IL)-6, IL-8, IL-17, vascular endothelial growth factor (VEGF), sclerostin (SOST) and osteoprotegerin (OPG) were evaluated by qPCR analysis at baseline and 6-month follow-up. Changes in cytokine mRNA expression levels were analyzed and compared with clinical/radiographic parameters. Both decontamination methods lead to the downregulations of the selected gene expressions. Ozone group showed significantly higher clinical attachment level (CAL) and radiographic defect fill (DF) values at 6 months compared to the control group (p = 0.026 and p = 0.011). The downregulation of SOST levels was significantly associated with probing depth reduction and radiographic DF (p < 0.05). Implant surface decontamination procedures applied with the RST contribute to a notable reduction in immuno-inflammatory response. The additional use of ozone therapy could have favorable effects in anti-infective regimens of peri-implantitis therapy. SOST, which was found to have significant relationship with both clinical and radiographic outcomes, could be a valuable indicator for the progression of peri-implantitis and may aid the development of new therapeutic strategies for bone gain in the RST of peri-implantitis.
本试验旨在分析种植体表面去污程序与种植体周围炎的重建性外科治疗（RST）相结合对种植体周围龈沟液（PICF）中选定生物标志物基因表达水平的影响。40 名患有种植体周围炎的患者接受 RST+使用无菌生理盐水和臭氧疗法（臭氧组）或单独使用无菌生理盐水（对照组）对种植体表面进行去污治疗。在基线和 6 个月随访时，通过 qPCR 分析评估白细胞介素（IL）-6、IL-8、IL-17、血管内皮生长因子（VEGF）、硬化蛋白（SOST）和护骨素（OPG）的基因表达水平。分析并比较细胞因子 mRNA 表达水平与临床/影像学参数的变化。两种去污方法均导致所选基因表达下调。与对照组相比，臭氧组在 6 个月时的临床附着水平（CAL）和影像学缺损填充（DF）值显著更高（p=0.026 和 p=0.011）。SOST 水平的下调与探诊深度减少和影像学 DF 显著相关（p<0.05）。应用 RST 的种植体表面去污程序有助于显著降低免疫炎症反应。臭氧疗法的额外应用可能对种植体周围炎治疗的抗感染方案有有利影响。SOST 与临床和影像学结果均有显著关系，可能是种植体周围炎进展的一个有价值指标，并可能有助于开发用于 RST 中种植体周围骨获得的新治疗策略。
