College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China.
Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing 100048, China.
Molecules. 2019 May 2;24(9):1715. doi: 10.3390/molecules24091715.
In this study, effects of different pretreatment methods on the enzymatic digestibility of , a ubiquitous wild grass in China, were investigated to evaluate its potential as a feedstock for biofuel production. The stalk samples were separately pretreated with HSO, NaOH and FeCl solutions of different concentrations at 120 °C for 30 min, after which enzymatic hydrolysis was conducted to measure the digestibility of pretreated samples. Results demonstrated that different pretreatments were effective at removing hemicellulose, among which ferric chloride pretreatment (FCP) gave the highest soluble sugar recovery (200.2 mg/g raw stalk) from the pretreatment stage. In comparison with FCP and dilute acid pretreatment (DAP), dilute alkaline pretreatment (DALP) induced much higher delignification and stronger morphological changes of the biomass, making it more accessible to hydrolysis enzymes. As a result, DALP using 1.2% NaOH showed the highest total soluble sugar yield through the whole process from pretreatment to enzymatic hydrolysis (508.5 mg/g raw stalk). The present work indicates that DALP and FCP have the potential to enhance the effective bioconversion of lignocellulosic biomass like , hence making this material a valuable and promising energy plant.
本研究旨在探讨不同预处理方法对中国广泛分布的野生草本植物 的酶解消化率的影响，以评估其作为生物燃料生产原料的潜力。将秸秆样品分别用不同浓度的 HSO、NaOH 和 FeCl 溶液在 120°C 下预处理 30 分钟，然后进行酶水解以测量预处理样品的消化率。结果表明，不同的预处理方法在去除半纤维素方面均有效，其中三氯化铁预处理（FCP）从预处理阶段获得的可溶性糖回收率最高（200.2mg/g 原秸秆）。与 FCP 和稀酸预处理（DAP）相比，稀碱预处理（DALP）诱导了更高的木质素脱除和更强的生物质形态变化，使其更易于水解酶作用。因此，使用 1.2%NaOH 的 DALP 在从预处理到酶水解的整个过程中表现出最高的总可溶性糖产率（508.5mg/g 原秸秆）。本研究表明，DALP 和 FCP 有可能增强木质纤维素生物质如 的有效生物转化，因此使该材料成为一种有价值和有前途的能源植物。
